cDNA microarray analysis of individual Duchenne muscular dystrophy patients

被引：57
作者：
Noguchi, S
Tsukahara, T
Fujita, M
Kurokawa, R
Tachikawa, M
Toda, T
Tsujimoto, A
Arahata, K
Nishino, I
机构：
[1] Natl Ctr Neurol & Psychiat, Natl Inst Neurosci, Dept Neuromusc Res, Kodaira, Tokyo 1878502, Japan
[2] Japan Sci & Technol Corp, CREST, Kawaguchi, Saitama 3320012, Japan
[3] Osaka Univ, Grad Sch Med, Dept Post Genomics & Dis, Div Funct Genomics, Suita, Osaka 5650871, Japan
[4] DNA Chip Res Inc, Yokohama, Kanagawa 2300045, Japan
来源：
HUMAN MOLECULAR GENETICS | 2003年 / 12卷 / 06期
关键词：
D O I：
10.1093/hmg/ddg065
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We have developed a novel cDNA microarray encompassing 3500 genes expressed in skeletal muscle. With this system, we have performed the first study of gene expression in samples from individual patients. We analyzed muscle specimen from individuals with Duchenne muscular dystrophy to identify differences among patients. Among the variably expressed genes, we focused on the expression of the genes encoding HLA-related proteins, myosin light chains and troponin Ts as markers of muscle necrosis and regeneration. The expression patterns of these genes correlated with the severity of dystrophic changes on histological examination. Our cDNA microarray provides a new tool to investigate molecular muscle pathology.
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收藏
页码：595 / 600
页数：6
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