Biocatalytic Synthesis of Dihydroxynaphthoic Acids by Cytochrome P450 CYP199A2

被引：14

作者：

Furuya, Toshiki ^{[1
]}

Kino, Kuniki ^{[1
]}

机构：

[1] Waseda Univ, Dept Appl Chem, Fac Sci & Engn, Shinjuku Ku, Tokyo 1698555, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2009年 / 73卷 / 12期

关键词：

CYP199A2; cytochrome P450; hydroxylation; naphthoic acid; oxidation; RHODOPSEUDOMONAS-PALUSTRIS; BIOSYNTHETIC-PATHWAY; MONOOXYGENASES; MENAQUINONE; INHIBITORS; ENZYMES;

D O I：

10.1271/bbb.90624

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

CYP199A2, a bacterial P450 monooxygenase from Rhodopseudomonas palustris, was found to exhibit oxidation activity towards three hydroxynaphthoic acids. Whole cells of the recombinant Escherichia coli strain expressing CYP199A2 efficiently catalyzed the regioselective oxidation of 1-, 3-, and 6-hydroxy-2-naphthoic acids to produce 1,7-, 3,7-, and 6,7-dihydroxynaphthoic acid respectively. These results suggest that CYP199A2 might be a useful oxidation biocatalyst for the synthesis of dihydroxynaphthoic acids.

引用

页码：2796 / 2799

页数：4

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