DNA extraction using bacterial magnetic particles modified with hyperbranched polyamidoamine dendrimer

被引：97

作者：

Yoza, B ^{[1
]}

Arakaki, A ^{[1
]}

Matsunaga, T ^{[1
]}

机构：

[1] Tokyo Univ Agr & Technol, Dept Biotechnol, Tokyo 1848588, Japan

来源：

JOURNAL OF BIOTECHNOLOGY | 2003年 / 101卷 / 03期

关键词：

bacterial magnetite; dendrimer; hyperbranched; polyamidoamine; DNA extraction;

D O I：

10.1016/S0168-1656(02)00342-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A cascading hyperbranched polyamidoamine dendrimer was synthesized on the surface of bacterial magnetite from Magnetospirillum magneticum AMB-1 to allow enhanced extraction of DNA from fluid suspensions. Characterization of the synthesis revealed linear doubling of the surface amine charge from generations one through five starting with an amino silane initiator. Furthermore, transmission electron microscopy revealed clear dispersion of the single domain magnetite in aqueous solution. The dendrimer modified magnetic particles have been used to carry out magnetic separation of DNA. Binding and release efficiencies increased with the number of generations and those of bacterial magnetite modified with six generation dendrimer were 7 and 11 times respectively as many as those of bacterial magnetite modified with only amino silane. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：219 / 228

页数：10

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