Stereoselective effects of ibuprofen in adult zebrafish (Danio rerio) using UPLC-TOF/MS-based metabolomics

被引：46

作者：

Song, Yue ^{[1
]}

Chai, Tingting ^{[1
,2
]}

Yin, Zhiqiang ^{[1
]}

Zhang, Xining ^{[1
]}

Zhang, Wei ^{[1
]}

Qian, Yongzhong ^{[1
]}

Qiu, Jing ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Key Lab Agroprod Qual & Safety, Key Lab Agrifood Qual & Safety,Minist Agr, Beijing 100081, Peoples R China

[2] Zhejiang A&F Univ, Coll Agr & Food Sci, Key Lab Qual Improvement Agr Prod Zhejiang Prov, Linan 311300, Zhejiang, Peoples R China

来源：

ENVIRONMENTAL POLLUTION | 2018年 / 241卷

基金：

中国国家自然科学基金;

关键词：

Ibuprofen; Stereoselectivity; Toxicity; Zebrafish; Metabolomics; PERSONAL CARE PRODUCTS; OXIDATIVE-STRESS; MASS-SPECTROMETRY; ENERGY-METABOLISM; NERVOUS-SYSTEM; AMINO-ACIDS; L-CYSTEINE; PHARMACEUTICALS; WATER; EXPOSURE;

D O I：

10.1016/j.envpol.2018.06.009

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Ibuprofen (IBU), as a commonly used non-steroidal anti-inflammatory drug (NSAID) and pharmaceutical and personal care product (PPCP), is frequently prescribed by doctors to relieve pain. It is widely released into environmental water and soil in the form of chiral enantiomers by the urination and defecation of humans or animals and by sewage discharge from wastewater treatment plants. This study focused on the alteration of metabolism in the adult zebrafish (Danio rerio) brain after exposure to R-(-)-/S-(+)-/racIBU at 5 mu g L-1 for 28 days. A total of 45 potential biomarkers and related pathways, including amino acids and their derivatives, purine and its derivatives, nucleotides and other metabolites, were observed with untargeted metabolomics. To validate the metabolic disorders induced by IBU, 22 amino acids and 3 antioxidant enzymes were selected to be quantitated and determined using targeted metabolomics and enzyme assay. Stereoselective changes were observed in the 45 identified biomarkers from the untargeted metabolomics analysis. The 22 amino acids quantitated in targeted metabolomics and 3 antioxidant enzymes determined in enzyme assay also showed stereoselective changes after R-(-)-/S-(+)-/racIBU exposure. Results showed that even at a low concentration of R-(-)-/S-(+)-/rac-IBU, disorders in metabolism and antioxidant defense systems were still induced with stereoselectivity. Our study may enable a better understanding of the risks of chiral PPCPs in aquatic organisms in the environment. (C) 2018 Elsevier Ltd. All rights reserved.

引用

页码：730 / 739

页数：10

共 76 条

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