Efficient induction of flower-color mutants by ion beam irradiation in petunia seedlings treated with high sucrose concentration

被引:28
作者
Hase, Yoshihiro [1 ]
Okamura, Masachika [2 ]
Takeshita, Daigaku [2 ]
Narumi, Issay [1 ]
Tanaka, Atsushi [1 ]
机构
[1] Japan Atom Energy Agcy, Quantum Beam Sci Directorate, Takasaki, Gunma 3701292, Japan
[2] Kirin Agribio Co Ltd, Plant Res Ctr, Sakura, Tochigi 3291414, Japan
关键词
Flower color; ion beam; mutation; petunia; sucrose; DOUBLE-STRAND BREAKS; CHROMATIN-STRUCTURE; ARABIDOPSIS-THALIANA; CHALCONE SYNTHASE; ANTHOCYANIN; GENE; DNA; BIOSYNTHESIS; EXPRESSION; MUTATION;
D O I
10.5511/plantbiotechnology.27.99
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We examined the effect of pretreatment on the frequency of flower-color mutants induced by ion beams. We found that petunia seedlings treated with 3% sucrose from 8 days after sowing accumulated significant amount of pigments within 4 days compared to non-treated control seedlings. The petunia seedlings treated with sucrose were exposed to 320-MeV carbon ions. The sucrose treatment did not affect the survival rate and seed fertility of the M-1 plants. In the M-2 lines obtained by self-pollination of individual M-1 plants, chlorophyll mutants were obtained in both treated and non-treated groups with a similar frequency. Flower-color mutants that included magenta, purple, light pink and white were obtained from the original violet color. The frequency of flower-color mutants was significantly higher in the sucrose-treated group than in the non-treated group. These results suggest that sucrose pretreatment specifically increases the frequency of flower-color mutation following ion beam irradiation.
引用
收藏
页码:99 / 103
页数:5
相关论文
共 24 条
[1]   Light-induced vegetative anthocyanin pigmentation in Petunia [J].
Albert, Nick W. ;
Lewis, David H. ;
Zhang, Huaibi ;
Irving, Louis J. ;
Jameson, Paula E. ;
Davies, Kevin M. .
JOURNAL OF EXPERIMENTAL BOTANY, 2009, 60 (07) :2191-2202
[2]   MYBL2 is a new regulator of flavonoid biosynthesis in Arabidopsis thaliana [J].
Dubos, Christian ;
Le Gourrierec, Jose ;
Baudry, Antoine ;
Huep, Gunnar ;
Lanet, Elodie ;
Debeaujon, Isabelle ;
Routaboul, Jean-Marc ;
Alboresi, Alessandro ;
Weisshaar, Bernd ;
Lepiniec, Loic .
PLANT JOURNAL, 2008, 55 (06) :940-953
[3]  
ELIA MC, 1992, CANCER RES, V52, P1580
[4]   Chromatin structure influences the sensitivity of DNA to γ-radiation [J].
Falk, Martin ;
Lukasova, Emilie ;
Kozubek, Stanislav .
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2008, 1783 (12) :2398-2414
[5]  
Griesbach RJ, 1996, J HERED, V87, P241, DOI 10.1093/oxfordjournals.jhered.a022993
[6]   Enhancement of anthocyanin biosynthesis by sugar in radish (Raphanus sativus) hypocotyl [J].
Hara, M ;
Oki, K ;
Hoshino, K ;
Kuboi, T .
PLANT SCIENCE, 2003, 164 (02) :259-265
[7]   A mutation in the uvi4 gene promotes progression of endo-reduplication and confers increased tolerance towards ultraviolet B light [J].
Hase, Y ;
Trung, KH ;
Matsunaga, T ;
Tanaka, A .
PLANT JOURNAL, 2006, 46 (02) :317-326
[8]   Heavy-ion-induced mutants in sweet pepper isolated by M1 plant selection [J].
Honda, Ichiro ;
Kikuchi, Kaori ;
Matsuo, Satoshi ;
Fukuda, Machiko ;
Saito, Hiroyuki ;
Ryuto, Hiromichi ;
Fukunishi, Nobuhisa ;
Abe, Tomoko .
EUPHYTICA, 2006, 152 (01) :61-66
[9]   CLONING AND MOLECULAR CHARACTERIZATION OF THE CHALCONE SYNTHASE MULTIGENE FAMILY OF PETUNIA-HYBRIDA [J].
KOES, RE ;
SPELT, CE ;
VANDENELZEN, PJM ;
MOL, JNM .
GENE, 1989, 81 (02) :245-257
[10]  
Kuboi T., 2004, PLANT BIOTECHNOL, V21, P401, DOI [10.5511/plantbiotechnology.21.401, DOI 10.5511/plantbiotechnology.21.401]