Cytotoxic effects of a chlorhexidine mouthwash and of an enzymatic mouthwash on human gingival fibroblasts

被引：43

作者：

Coelho, Ana Sofia ^{[1
,2
,3
,4
,5
]}

Laranjo, Mafalda ^{[2
,3
,4
,5
,6
]}

Goncalves, Ana Cristina ^{[2
,3
,4
,5
,6
]}

Paula, Anabela ^{[1
,2
,3
,4
,5
]}

Paulo, Siri ^{[1
,2
,3
,4
,5
,7
]}

Abrantes, Ana Margarida ^{[2
,3
,4
,5
,6
]}

Caramelo, Francisco ^{[2
,3
,4
,5
,8
]}

Ferreira, Manuel Marques ^{[1
,2
,3
,4
,5
,7
]}

Silva, Mario Jorge ^{[9
]}

Carrilho, Eunice ^{[1
,2
,3
,4
,5
]}

Botelho, Maria Filomena ^{[2
,3
,4
,5
,6
]}

机构：

[1] Univ Coimbra, Fac Med, Inst Integrated Clin Practice, Area Med Dent, Av Bissaya Barreto,Bloco Celas, P-3000075 Coimbra, Portugal

[2] Univ Coimbra, Fac Med, Coimbra Inst Clin & Biomed Res iCBR, Area Med Dent, Av Bissaya Barreto,Bloco Celas, P-3000075 Coimbra, Portugal

[3] Univ Coimbra, Ctr Innovat Biomed & Biotechnol, Area Med Dent, Av Bissaya Barreto,Bloco Celas, P-3000075 Coimbra, Portugal

[4] Univ Coimbra, Fac Med, CIMAGO Ctr Invest Envirom Genet & Oncobiol, Area Med Dent, Av Bissaya Barreto,Bloco Celas, P-3000075 Coimbra, Portugal

[5] Univ Coimbra, CNC IBILI, Area Med Dent, Av Bissaya Barreto,Bloco Celas, P-3000075 Coimbra, Portugal

[6] Univ Coimbra, Fac Med, Biophys Inst, Area Med Dent, Av Bissaya Barreto,Bloco Celas, P-3000075 Coimbra, Portugal

[7] Univ Coimbra, Fac Med, Endodont Inst, Area Med Dent, Av Bissaya Barreto,Bloco Celas, P-3000075 Coimbra, Portugal

[8] Univ Coimbra, Fac Med, Lab Biostat & Med Informat, Area Med Dent, Av Bissaya Barreto,Bloco Celas, P-3000075 Coimbra, Portugal

[9] Univ Porto, Fac Dent Med, Oporto, Portugal

来源：

ODONTOLOGY | 2020年 / 108卷 / 02期

关键词：

Chlorhexidine; Mouthwash; Enzymatic mouthwash; Cytotoxicity; Fibroblasts; SODIUM-HYPOCHLORITE; IN-VITRO; LACTOPEROXIDASE; DIGLUCONATE; LYSOZYME; LACTOFERRIN; METABOLISM; ATTACHMENT; BACTERIA; EFFICACY;

D O I：

10.1007/s10266-019-00465-z

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

The aim of this study was to evaluate the cytotoxic effects of an enzymatic mouthwash and of a chlorhexidine mouthwash on human gingival fibroblasts. The metabolic activity of the fibroblasts exposed to each mouthwash was assessed by the MTT assay and the protein content was assessed by the SRB assay. The flow cytometry was used to evaluate the cell cycle and the types of cell death. The oxidative status was evaluated through the DCF and the DHE probes and the intracellular GSH concentration and the mitochondrial membrane potential through JC-1. The cytotoxicity of both mouthwashes was found to be dependent on the exposure time and on the concentration. However, the cytotoxicity of the enzymatic mouthwash was found to be lower than that of the chlorhexidine mouthwash. A trend towards increased oxidative stress was observed for both mouthwashes. After exposing the fibroblasts to the mouthwashes, a G2/M phase block was observed and cell death occurred predominantly by necrosis. The effects of chlorhexidine on fibroblasts were identified at lower concentrations than those used in clinical practice. Therefore, the use of chlorhexidine as an antiseptic in surgical and postoperative situations should be limited. In order to clarify the clinical significance of the enzymatic mouthwash cytotoxicity new clinical studies will be necessary.

引用

页码：260 / 270

页数：11

共 38 条

[1] Evaluation of the clinical efficacy of BiotSne Oral Balance in patients with secondary Sjogren's syndrome: a pilot study [J].