Generation of polyclonal antibodies against multiple proteins in a single rabbit and subsequent isolation of the specific immunoglobulins as a tool for proteomics research

被引：3
作者：
Jacquot, JP
Rouhier, N
Navrot, N
Decottignies, JP
机构：
[1] Univ Henri Poincare, UMR 1136, INRA, Fac Sci, F-54506 Vandoeuvre Les Nancy, France
[2] UTSL Lille Flandres Artois, Chim Biol Lab, F-59650 Villeneuve Dascq, France
来源：
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY | 2004年 / 40卷
关键词：
affinity chromatography; immunization; immunoglobulins; immunopurification; polyclonal antibodies;
D O I：
10.1042/BA20030196
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A single rabbit has been immunized with a mixture of six different proteins: green fluorescent protein, ferredoxin:NADP oxidoreductase, NADPH:thioredoxin reductase, thioredoxin f, fructose bisphosphatase and cyclophilin. Individual IgGs were re-purified from the serum through specific interaction on small columns generated by grafting the antigen on a CNBr-Sepharose matrix. The specificity of the isolated IgG was checked by Western-blot analysis. Purified and specific IgG fractions were obtained with all antigens except thioredoxin f.
引用
收藏
页码：181 / 185
页数：5
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