Structural insights into preinitiation complex assembly on core promoters

被引：127

作者：

Chen, Xizi ^{[1
,2
]}

Qi, Yilun ^{[1
,2
]}

Wu, Zihan ^{[1
,2
]}

Wang, Xinxin ^{[1
,2
]}

Li, Jiabei ^{[1
,2
]}

Zhao, Dan ^{[1
,2
]}

Hou, Haifeng ^{[1
,2
]}

Li, Yan ^{[1
,2
]}

Yu, Zishuo ^{[1
,2
]}

Liu, Weida ^{[1
,2
]}

Wang, Mo ^{[1
,2
]}

Ren, Yulei ^{[1
,2
]}

Li, Ze ^{[1
,2
]}

Yang, Huirong ^{[1
,2
]}

Xu, Yanhui ^{[1
,2
,3
,4
,5
]}

机构：

[1] Fudan Univ, Shanghai Canc Ctr, Inst Biomed Sci, State Key Lab Genet Engn, Shanghai 200032, Peoples R China

[2] Fudan Univ, Shanghai Key Lab Med Epigenet, Shanghai Med Coll, Shanghai 200032, Peoples R China

[3] Minist Sci & Technol, Int Colab Med Epigenet & Metab, Beijing, Peoples R China

[4] Fudan Univ, Dept Syst Biol Med, Sch Basic Med Sci, Shanghai Med Coll, Shanghai 200032, Peoples R China

[5] Fudan Univ, Sch Life Sci, Collaborat Innovat Ctr Genet & Dev, Human Phenome Inst, Shanghai 200433, Peoples R China

来源：

SCIENCE | 2021年 / 372卷 / 6541期

基金：

国家重点研发计划; 中国国家自然科学基金;

关键词：

RNA-POLYMERASE-II; TATA-BINDING-PROTEIN; TRANSCRIPTION FACTORS IIE; PRE-INITIATION COMPLEX; CDK-ACTIVATING KINASE; C-TERMINAL DOMAIN; CRYSTAL-STRUCTURE; FACTOR TFIIH; DNA-REPAIR; CYCLIN H;

D O I：

10.1126/science.aba8490

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Transcription factor IID (TFIID) recognizes core promoters and supports preinitiation complex (PIC) assembly for RNA polymerase II (Pol II)-mediated eukaryotic transcription. We determined the structures of human TFIID-based PIC in three stepwise assembly states and revealed two-track PIC assembly: stepwise promoter deposition to Pol II and extensive modular reorganization on track I (on TATA-TFIID-binding element promoters) versus direct promoter deposition on track II (on TATA-only and TATA-less promoters). The two tracks converge at an similar to 50-subunit holo PIC in identical conformation, whereby TFIID stabilizes PIC organization and supports loading of cyclin-dependent kinase (CDK)activating kinase (CAK) onto Pol II and CAK-mediated phosphorylation of the Pol II carboxyl-terminal domain. Unexpectedly, TBP of TFIID similarly bends TATA box and TATA-less promoters in PIC. Our study provides structural visualization of stepwise PIC assembly on highly diversified promoters.

引用

页码：480 / +

页数：90

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