Tannin Nanoparticles (NP99) Enhances the Anticancer Effect of Tamoxifen on ER+ Breast Cancer Cells

被引：10

作者：

AlMalki, Faizah A. ^{[1
]}

Hassan, Aziza M. ^{[2
]}

Klaab, Zeinab M. ^{[1
]}

Abdulla, Soliman ^{[3
]}

Pizzi, Antonio ^{[4
]}

机构：

[1] Taif Univ, Coll Sci, Dept Biol, At Taif 21944, Saudi Arabia

[2] Taif Univ, Coll Sci, Dept Biotechnol, At Taif 21944, Saudi Arabia

[3] Alexandria Univ, Fac Sci, Dept Phys, Alexandria, Egypt

[4] Univ Lorraine, ENSTIB LERMAB, F-88051 Epinal, France

来源：

JOURNAL OF RENEWABLE MATERIALS | 2021年 / 9卷 / 12期

关键词：

Condensed tannin; nanoparticles; tamoxifen; MCF-7; cells; anticancer; ENDOCRINE THERAPY; APOPTOSIS; MCF-7; ACID; MECHANISMS; ARREST; COLON;

D O I：

10.32604/jrm.2021.016173

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Recently, natural substances in the form of nanoparticles are increasingly being used in different field, particularly in medicines to enhance their beneficial effects in treatment and prevention. Cancer cells of the breast (MCF-7) have been chosen to be examined and treated in vitro with conventional drug Tamoxifen (Tam) and tannin nanoparticles extract (NP99) individually or in combination. MTT reagent has been applied to assess the cell viability and propagation percentage, DNA fragmentation and mRNA relative expression of apoptotic genes to study the cell death pathway. The results showed that Tam and tannin NP99 triggered cytotoxic activity towards the MCF-7 cell. They reduce the viability and induced high potent repressive activity on cell proliferation percentage and induced apoptosis as indicated by rising the percentage in DNA fragmentation. Effect of NP99 extract exhibited its effect in a dose and time-varying. The combined treatment of Tam and NP99 were much more efficient than individual drugs. It could be concluded that NP99 is considered a promising natural anticancer agent as a new tool in therapeutic strategies.

引用

页码：2077 / 2092

页数：16

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