Next generation sequencing for miRNA profile of spleen CD4+ T cells in the murine model of acute asthma

被引：12

作者：

Liu, Ye ^{[1
]}

Chen, Zhongqi ^{[2
]}

Xu, Kun ^{[3
]}

Wang, Zhengxia ^{[2
]}

Wu, Chaojie ^{[2
]}

Sun, Zhixiao ^{[2
]}

Ji, Ningfei ^{[1
,2
]}

Huang, Mao ^{[2
]}

Zhang, Mingshun ^{[4
]}

机构：

[1] Jiangsu Prov Geriatr Hosp, Dept Geriatr, Nanjing 210024, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Dept Resp & Crit Care Med, Affiliated Hosp 1, Nanjing 210029, Jiangsu, Peoples R China

[3] Nanjing Med Univ, Affiliated Wuxi Peoples Hosp 2, Dept Resp Med, Wuxi 214002, Peoples R China

[4] Nanjing Med Univ, Dept Immunol, Nanjing 211166, Jiangsu, Peoples R China

来源：

EPIGENOMICS | 2018年 / 10卷 / 08期

基金：

中国国家自然科学基金;

关键词：

asthma; CD4(+) T cells; miRNAs; next generation sequencing; Th2; MICRORNA TARGET PREDICTION; DIFFERENTIAL EXPRESSION; INFLAMMATION; TOOL;

D O I：

10.2217/epi-2018-0043

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Aim: To explore the miRNAs profile of CD4(+) T lymphocytes in asthma via next generation sequencing. Methods: In the murine model of acute asthma, spleen CD4(+) T lymphocytes were sorted, in which small RNAs were extracted and sequenced. Novel miRNAs were measured with real time quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results: A total of 127 miRNAs were found to exhibit at least twofold change. In the 262 predicted novel miRNAs, 14 novel miRNAs were measured in qRT-PCR in the sorted CD4(+) T cells or in the differentiated Th1/Th2 cells and novel miR-11 (xxx-m0228-3p) was significantly decreased in the sorted CD4(+) T cells from the murine model of asthma and in the Th2 cells. Conclusion: Aberrant miRNAs profile in the CD4(+) T lymphocytes from acute asthma was documented.

引用

页码：1071 / 1083

页数：13

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