A concise guide to cDNA microarray analysis

被引：634

作者：

Hegde, P ^{[1
]}

Qi, R ^{[1
]}

Abernathy, K ^{[1
]}

Gay, C ^{[1
]}

Dharap, S ^{[1
]}

Gaspard, R ^{[1
]}

Hughes, JE ^{[1
]}

Snesrud, E ^{[1
]}

Lee, N ^{[1
]}

Quackenbush, J ^{[1
]}

机构：

[1] Inst Genom Res, Rockville, MD 20850 USA

来源：

BIOTECHNIQUES | 2000年 / 29卷 / 03期

关键词：

D O I：

10.2144/00293bi01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microarray expression analysis has become one of the most widely used functional genomics tools. Efficient application of this technique requires the development of robust and reproducible protocols. We have optimized all aspects of the process, including PCR amplification of target cDNA clones, microarray printing, probe labeling and hybridization, and have developed strategies for data normalization and analysis.

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页码：548 / +

页数：10

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