Construction of a tissue-specific transcription factor-tethered extracellular matrix protein via coiled-coil helix formation

被引：9

作者：

Siew, SokeLee ^{[1
]}

Kaneko, Mami ^{[1
]}

Mie, Masayasu ^{[1
]}

Kobatake, Eiry ^{[1
]}

机构：

[1] Tokyo Inst Technol, Interdisciplinary Grad Sch Sci & Engn, Dept Environm Chem & Engn, Midori Ku, 4259 Nagatsuta, Yokohama, Kanagawa 2268502, Japan

来源：

JOURNAL OF MATERIALS CHEMISTRY B | 2016年 / 4卷 / 14期

基金：

日本学术振兴会;

关键词：

GROWTH-FACTOR; TRANSDUCTION DOMAIN; DEFINED FACTORS; MYOGENIC DIFFERENTIATION; DIRECT CONVERSION; MYOD PROTEIN; CELLS; FIBROBLASTS; BIOMATERIALS; MYOBLASTS;

D O I：

10.1039/c5tb01579k

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

Tissue-specific transcription factors are key regulators of cellular differentiation. Previously, we succeeded in introducing basic helix-loop-helix tissue-specific transcription factor proteins into cells to induce cellular differentiation. Based on these results, we decided to focus on the use of tissue-specific transcription factor proteins in the construction of biomaterials. In this proof-of-concept study, we demonstrate the construction of a tissue-specific transcription factor-tethered extracellular matrix protein. Here, the tissue-specific transcription factor Olig2 was tethered to a designed artificial extracellular matrix protein via coiled-coil helix formation. Tethered Olig2 was introduced into mouse embryonic carcinoma P19 cells attached to our designed extracellular matrix protein, and was shown to exhibit the ability to induce neural differentiation.

引用

页码：2512 / 2518

页数：7

共 36 条

[1] The promotion of angiogenesis by growth factors integrated with ECM proteins through coiled-coil structures [J].