Conjugative DNA metabolism in Gram-negative bacteria

被引：266

作者：

de la Cruz, Fernando ^{[2
]}

Frost, Laura S. ^{[1
]}

Meyer, Richard J. ^{[3
]}

Zechner, Ellen L. ^{[4
]}

机构：

[1] Univ Alberta, Dept Biol Sci, Edmonton, AB T6G 2E9, Canada

[2] Univ Cantabria, Dept Mol Biol, E-39005 Santander, Spain

[3] Univ Texas Austin, Dept Mol Genet & Microbiol, Austin, TX 78712 USA

[4] Graz Univ, Inst Mol Enzymol, Graz, Austria

来源：

FEMS MICROBIOLOGY REVIEWS | 2010年 / 34卷 / 01期

基金：

奥地利科学基金会;

关键词：

relaxosome; relaxase; mobilization; conjugation; coupling protein; origin of transfer; COMPLETE NUCLEOTIDE-SEQUENCE; SINGLE-STRANDED-DNA; PLASMID-ENCODED PRODUCTS; TRAI HELICASE ACTIVITY; F-FACTOR TRAY; ESCHERICHIA-COLI; TRANSFER ORIGIN; MUTATIONAL ANALYSIS; TRANSFER REGION; GENE-PRODUCT;

D O I：

10.1111/j.1574-6976.2009.00195.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacterial conjugation in Gram-negative bacteria is triggered by a signal that connects the relaxosome to the coupling protein (T4CP) and transferosome, a type IV secretion system. The relaxosome, a nucleoprotein complex formed at the origin of transfer (oriT), consists of a relaxase, directed to the nic site by auxiliary DNA-binding proteins. The nic site undergoes cleavage and religation during vegetative growth, but this is converted to a cleavage and unwinding reaction when a competent mating pair has formed. Here, we review the biochemistry of relaxosomes and ponder some of the remaining questions about the nature of the signal that begins the process.

引用

页码：18 / 40

页数：23

共 173 条

[1] SPECIFIC DNA-BINDING OF THE TRAM PROTEIN TO THE ORIT REGION OF PLASMID R100 [J].

ABO, T ;

INAMOTO, S ;

OHTSUBO, E .

JOURNAL OF BACTERIOLOGY, 1991, 173 (20) :6347-6354

[2] CHARACTERIZATION OF THE FUNCTIONAL SITES IN THE ORIT REGION INVOLVED IN DNA TRANSFER PROMOTED BY SEX FACTOR PLASMID R100 [J].

ABO, T ;

OHTSUBO, E .

JOURNAL OF BACTERIOLOGY, 1995, 177 (15) :4350-4355

[3] Mobilization of the incQ plasmid R300B with a chromosomal conjugation system in Salmonella enterica serovar Typhi [J].

Baker, Stephen ;

Pickard, Derek ;

Whitehead, Sally ;

Farrar, Jeremy ;

Dougan, Gordon .

JOURNAL OF BACTERIOLOGY, 2008, 190 (11) :4084-4087

[4] Relaxed specificity of the R1162 nickase: a model for evolution of a system for conjugative mobilization of plasmids [J].

Becker, EC ;

Meyer, RJ .

JOURNAL OF BACTERIOLOGY, 2003, 185 (12) :3538-3546

[5] MobA, the DNA strand transferase of plasmid R1162 - The minimal domain required for DNA processing at the origin of transfer [J].

Becker, EC ;

Meyer, RJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (17) :14575-14580

[6] CONJUGATIVE TRANSFER BY THE VIRULENCE SYSTEM OF AGROBACTERIUM-TUMEFACIENS [J].

BEIJERSBERGEN, A ;

DENDULKRAS, A ;

SCHILPEROORT, RA ;

HOOYKAAS, PJJ .

SCIENCE, 1992, 256 (5061) :1324-1327

[7] Thirty-eight C-terminal amino acids of the coupling protein TraD of the F-like conjugative resistance plasmid R1 are required and sufficient to confer binding to the substrate selector protein TraM [J].

Beranek, A ;

Zettl, M ;

Lorenzoni, K ;

Schauer, A ;

Manhart, M ;

Koraimann, G .

JOURNAL OF BACTERIOLOGY, 2004, 186 (20) :6999-7006

[8] SPECIFIC BINDING OF MOBA, A PLASMID-ENCODED PROTEIN INVOLVED IN THE INITIATION AND TERMINATION OF CONJUGAL DNA TRANSFER, TO SINGLE-STRANDED ORIT DNA [J].

BHATTACHARJEE, MK ;

MEYER, RJ .

NUCLEIC ACIDS RESEARCH, 1993, 21 (19) :4563-4568

[9] GENERAL ORGANIZATION OF THE CONJUGAL TRANSFER GENES OF THE INCW PLASMID R388 AND INTERACTIONS BETWEEN R388 AND INCN AND INCP PLASMIDS [J].

BOLLAND, S ;

LLOSA, M ;

AVILA, P ;

DELACRUZ, F .

JOURNAL OF BACTERIOLOGY, 1990, 172 (10) :5795-5802

[10] Structural comparison of the integrative and conjugative elements R391, pMERPH, R997, and SXT [J].

Böltner, D ;

Osborn, AM .

PLASMID, 2004, 51 (01) :12-23

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