Rapid estimation of numbers of fecal Bacteroidetes by use of a quantitative PCR assay for 16S rNA genes

被引：129

作者：

Dick, LK ^{[1
]}

Field, KG ^{[1
]}

机构：

[1] Oregon State Univ, Dept Microbiol, Corvallis, OR 97331 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 09期

关键词：

D O I：

10.1128/AEM.70.9.5695-5697.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Assessment of health risk associated with fecal pollution requires a reliable fecal indicator and a rapid quantification method. We report the development of a Taq nuclease assay for enumeration of 16S rRNA genes of Bacteroidetes. Sensitivity and correlation with standard fecal indicators provide experimental evidence for application of the assay in monitoring fecal pollution.

引用

页码：5695 / 5697

页数：3

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