Isolation, Identification, and Anti-Inflammatory Activity of Polysaccharides of Typha angustifolia

被引：32

作者：

Wei, Huan ^{[1
]}

Shi, Yuqi ^{[2
]}

Yuan, Zhixiang ^{[2
]}

Huang, Zhinan ^{[1
]}

Cai, Fuhong ^{[1
]}

Zhu, Jingfeng ^{[1
]}

Zhang, Wanwan ^{[1
]}

Li, Jia ^{[1
]}

Xiong, Qingping ^{[1
]}

Wang, Yunpeng ^{[1
]}

Wang, Xiaoli ^{[1
]}

机构：

[1] Huaiyin Inst Technol, Coll Life Sci & Food Engn, Jiangsu Prov Key Construct Lab Probiot Preparat, Huaian 223003, Peoples R China

[2] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Peoples R China

来源：

BIOMACROMOLECULES | 2021年 / 22卷 / 06期

基金：

中国国家自然科学基金;

关键词：

STRUCTURAL-CHARACTERIZATION; SULFATED POLYSACCHARIDE; ANTIOXIDANT ACTIVITIES; IMMUNOLOGICAL ACTIVITY; DEPROTEINIZATION; ELUCIDATION; ACTIVATION; P65;

D O I：

10.1021/acs.biomac.1c00235

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study aimed to purify, structurally characterize, and evaluate the anti-inflammatory activity of the polysaccharide extracted from Typha angustifolia. Two purified polysaccharides (PTA-1 and PTA-2) were obtained via DEAE-52 cellulose chromatography. Their structural characterizations and antioxidant activity were in vitro analyzed. To evaluate the anti-inflammatory activity of PTA-2, the levels of inflammatory cytokines, intracellular ROS production, and the inhibitory effects of the transcriptional activation of the nuclear factor kappa B (NF-kappa B) signaling pathway were determined. PTA-1 comprises glucose (100%) with alpha-(1 -> 3) glycosidic bonds, and PTA-2 comprises glucose (66.7%) and rhamnose (33.3%) formed by beta-(1 -> 3 glycosidic bonds. PTA-1 and PTA-2 showed strong antioxidant activity in vitro. Moreover, PTA-2 intervention (50, 100, and 200 mu g,/mL) suppressed the production of inflammatory cytokines, the activation of NF-kappa B signaling, and reactive oxygen species production significantly. The results identified PTA-2 as a natural product that could be applied in anti-inflammatory drugs.

引用

页码：2451 / 2459

页数：9

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