Quantitative intracellular calcium sensing with two-photon fluorescence lifetime imaging microscopy

被引：0
作者：
Kuwana, Eddy [1 ]
Vargas-Adams, Gracie [1 ]
Pappas, Todd C. [1 ]
Liopo, Anton V. [1 ]
Motamedi, Massoud [1 ]
机构：
[1] Ctr Biomed Engn, 301 Univ Blvd, Galveston, TX 77555 USA
来源：
PHOTONIC THERAPEUTICS AND DIAGNOSTICS III | 2007年 / 6424卷
关键词：
calcium sensing; fluorescence lifetime imaging; two-photon excitation;
D O I：
10.1117/12.723959
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
In this work, we demonstrate the use of Two-Photon Fluorescence Lifetime Imaging Microscopy (TP-FLIM) for intracellular calcium ([Ca2+](i)) measurement with a calcium sensitive fluorescent dye in a neuronal cell model and brain tissue. Calcium perturbations were induced via chemical stimulation and our results signify the potential of TP-FLIM for quantitative ([Ca2+](i)) measurement within the physiological range of intracellular [Ca2+].
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