The Supportive Role of Insulin-Like Growth Factor-I in the Differentiation of Murine Mesenchymal Stem Cells into Corneal-Like Cells

被引:17
|
作者
Trosan, Peter [1 ,2 ,3 ]
Javorkova, Eliska [1 ,2 ]
Zajicova, Alena [1 ]
Hajkova, Michaela [1 ,2 ]
Hermankova, Barbora [1 ,2 ]
Kossl, Jan [1 ,2 ]
Krulova, Magdalena [1 ,2 ]
Holan, Vladimir [1 ,2 ]
机构
[1] Acad Sci Czech Republ, Inst Expt Med, Dept Transplantat Immunol, Prague, Czech Republic
[2] Charles Univ Prague, Fac Sci, Dept Cell Biol, Prague 12808 2, Czech Republic
[3] Charles Univ Prague, Gen Univ Hosp Prague, Inst Inherited Metab Disorders, Fac Med 1,Lab Biol & Pathol Eye, Ke Karlovu 2, Prague 12808 2, Czech Republic
关键词
OCULAR SURFACE RECONSTRUCTION; BONE-MARROW; STROMAL CELLS; NANOFIBER SCAFFOLDS; LIMBAL; VITRO; TRANSPLANTATION; EPITHELIUM; MICROENVIRONMENT; REGENERATION;
D O I
10.1089/scd.2016.0030
中图分类号
Q813 [细胞工程];
学科分类号
摘要
This study was focused on characterizing the differentiation of bone marrow-derived mesenchymal stem cells (MSCs) into corneal-like cells. Mouse MSCs were isolated from the bone marrow, grown in cell culture for 3 weeks, and purified using a magnetic activated cell sorter. Purified MSCs were cultured with an extract prepared from excised corneas and in the presence or absence of insulin-like growth factor-I (IGF-I). Analysis by quantitative real-time polymerase chain reaction showed that the expression of corneal specific markers, such as cytokeratin 12 (K12), keratocan, and lumican, was already induced after a 3-day cultivation and gradually increased during the 10-day incubation of MSCs with the extract. The presence of IGF-I significantly increased differentiation. Immunofluorescence analysis of differentiated MSCs showed positive results for the K12 protein. The morphology of the differentiated cells and the expression of cell surface markers CD45, CD11b, CD73, CD44, and CD105 were comparable in the control and differentiated MSCs. Proliferative activity was even higher in differentiated cells than in untreated MSCs. Both untreated and differentiated MSCs inhibited the production of interleukin-2 and interferon-gamma in spleen cells stimulated with Concanavalin A. The results thus show that MSCs cultured in the presence of corneal extract and IGF-I efficiently differentiate into corneal-like cells. The differentiated cells possess characteristics of corneal epithelial cells and keratocytes, while at the same time maintaining MSC properties.
引用
收藏
页码:874 / 881
页数:8
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