Novel Method to Detect a Construct-Specific Sequence of the Acetolactate Synthase Gene in Genetically-Modified Flax CDC Triffid (FP967)

被引：12

作者：

Nakamura, Kosuke ^{[1
]}

Akiyama, Hiroshi ^{[1
]}

Yamada, Chihiro ^{[2
]}

Satoh, Rie ^{[1
]}

Makiyama, Daiki ^{[1
]}

Sakata, Kozue ^{[1
]}

Kawakami, Hiroshi ^{[2
]}

Mano, Junichi ^{[3
]}

Kitta, Kazumi ^{[3
]}

Teshima, Reiko ^{[1
]}

机构：

[1] Natl Inst Hlth Sci, Setagaya Ku, Tokyo 1588501, Japan

[2] Kyoritsu Womens Univ, Dept Food Sci & Nutr, Chiyoda Ku, Tokyo 1018437, Japan

[3] Natl Food Res Inst, Tsukuba, Ibaraki 3058642, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2010年 / 33卷 / 03期

关键词：

genetically modified organism; flax; polymerase chain reaction; genomic DNA; LINUM-USITATISSIMUM; RESISTANT;

D O I：

10.1248/bpb.33.532

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

During the fall of 2009, a trace of unauthorized genetically modified (GM) flax (Linum usitatissimum L.) line, CDC Triffid, which is resistant to sulfonylurea herbicides, was defected in many countries including Japan. A method to reliably identify, the CDC Triffid line was urgently required. We developed a novel construct-specific real-time polymerase chain reaction (PCR) method to identify the mutant acetolactate synthase gene in the CDC Triffid line. We confirmed that the method can detect 0.001% GM flax in DNA mixing solution. The study shows that the developed method is specific, sensitive and reliable way to monitor a trace of CDC Triffid.

引用

页码：532 / 534

页数：3

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