Development and characterization of twenty-five microsatellite markers for the longnose dace (Cyprinidae: Rhinichthys) using paired-end Illumina shotgun sequencing

被引：2

作者：

Beasley, Rochelle ^{[1
]}

Lance, Stacey L. ^{[1
]}

Ruskey, Jennifer A. ^{[2
,3
]}

Taylor, Eric B. ^{[2
,3
]}

机构：

[1] Univ Georgia, Savannah River Ecol Lab, Aiken, SC 29802 USA

[2] Univ British Columbia, Biodivers Res Ctr, Dept Zool, Vancouver, BC V6T 1Z4, Canada

[3] Univ British Columbia, Beaty Biodivers Museum, Vancouver, BC V6T 1Z4, Canada

来源：

CONSERVATION GENETICS RESOURCES | 2014年 / 6卷 / 04期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Rhinichthys; Microsatellite; PAL_FINDER; PCR primers; SSR;

D O I：

10.1007/s12686-014-0272-y

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

We isolated and characterized a total of 48 microsatellite loci in Rhinichthys cataractae a widely distributed freshwater fish that may contain several cryptic species. Loci were screened in 24 individuals from several areas of British Columbia, Canada. The number of alleles per locus ranged from 5 to 24, observed heterozygosity ranged from 0.250 to 0.940, and the probability of identity ranged from 0.006 to 0.453. These new loci are being used for conducting investigations into the genetic structure, diversity, and speciation in populations of this exceptionally broadly-distributed North American freshwater fish.

引用

页码：1011 / 1013

页数：3

共 3 条

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McPhail J.D., 2007, FRESHWATER FISHES BR

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