Genomic Epidemiology of NDM-1-Encoding Plasmids in Latin American Clinical Isolates Reveals Insights into the Evolution of Multidrug Resistance

被引:50
作者
Alejandro Marquez-Ortiz, Ricaurte [1 ,2 ]
Haggerty, Leanne [2 ,7 ]
Olarte, Narda [3 ]
Duarte, Carolina [4 ]
Garza-Ramos, Ulises [5 ]
Silva-Sanchez, Jesus [5 ]
Castro, Betsy E. [1 ]
Sim, Eby M. [2 ]
Beltran, Mauricio [4 ]
Moncada, Maria V. [1 ]
Valderrama, Alberto [3 ]
Castellanos, Jaime E. [6 ]
Charles, Ian G. [2 ,8 ]
Vanegas, Natasha [1 ,2 ]
Escobar-Perez, Javier [1 ]
Petty, Nicola K. [2 ]
机构
[1] Univ Bosque, Bacterial Mol Genet Lab, Bogota, DC, Colombia
[2] Univ Technol Sydney, ithree Inst, Sydney, NSW, Australia
[3] Tunal Hosp ESE, Bogota, Colombia
[4] Inst Nacl Salud, Grp Microbiol, Bogota, Colombia
[5] CISEI, INSP, Cuernavaca, Morelos, Mexico
[6] Univ Nacl Colombia, Grp Patogenesis Infecciosa, Bogota, DC, Colombia
[7] European Bioinformat Inst, Wellcome Trust Genome Campus, Cambridge, England
[8] Norwich Res Pk, Inst Food Res, Norwich, Norfolk, England
来源
GENOME BIOLOGY AND EVOLUTION | 2017年 / 9卷 / 06期
关键词
metallo-beta-lactamase; genomics; antibiotic resistance; Providencia rettgeri; mobile genetic elements; bacterial evolution; METALLO-BETA-LACTAMASE; KLEBSIELLA-PNEUMONIAE; ACINETOBACTER-BAUMANNII; PROVIDENCIA-RETTGERI; ANTIBIOTIC-RESISTANCE; BLA(NDM-1)-HARBORING PLASMID; BLA(NDM-1)-CARRYING PLASMID; CARBAPENEM RESISTANCE; CLASS-1; INTEGRONS; COMPLETE SEQUENCE;
D O I
10.1093/gbe/evx115
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Bacteria that produce the broad-spectrum Carbapenem antibiotic New Delhi Metallo-beta-lactamase (NDM) place a burden on health care systems worldwide, due to the limited treatment options for infections caused by them and the rapid global spread of this antibiotic resistance mechanism. Although it is believed that the associated resistance gene bla(NDM-1) originated in Acinetobacter spp., the role of Enterobacteriaceae in its dissemination remains unclear. In this study, we used whole genome sequencing to investigate the dissemination dynamics of blaNDM-1-positive plasmids in a set of 21 clinical NDM-1-positive isolates from Colombia and Mexico (Providencia rettgeri, Klebsiella pneumoniae, and Acinetobacter baumannii) as well as six representative NDM-1-positive Escherichia coli transconjugants. Additionally, the plasmids from three representative P. rettgeri isolates were sequenced by PacBio sequencing and finished. Our results demonstrate the presence of previously reported plasmids from K. pneumoniae and A. baumannii in different genetic backgrounds and geographically distant locations in Colombia. Three new previously unclassified plasmids were also identified in P. rettgeri from Colombia and Mexico, plus an interesting genetic link between NDM-1-positive P. rettgeri from distant geographic locations (Canada, Mexico, Colombia, and Israel) without any reported epidemiological links was discovered. Finally, we detected a relationship between plasmids present in P. rettgeri and plasmids from A. baumannii and K. pneumoniae. Overall, our findings suggest a Russian doll model for the dissemination of blaNDM-1 in Latin America, with P. rettgeri playing a central role in this process, and reveal new insights into the evolution and dissemination of plasmids carrying such antibiotic resistance genes.
引用
收藏
页码:1725 / 1741
页数:17
相关论文
共 100 条
  • [21] SHRiMP2: Sensitive yet Practical Short Read Mapping
    David, Matei
    Dzamba, Misko
    Lister, Dan
    Ilie, Lucian
    Brudno, Michael
    [J]. BIOINFORMATICS, 2011, 27 (07) : 1011 - 1012
  • [22] Characterization of the replication and mobilization regions of the multiresistance Klebsiella pneumoniae plasmid pJHCMW1
    Dery, KJ
    Chavideh, R
    Waters, V
    Chamorro, R
    Tolmasky, LS
    Tolmasky, ME
    [J]. PLASMID, 1997, 38 (02) : 97 - 105
  • [23] The Population Structure of Acinetobacter baumannii: Expanding Multiresistant Clones from an Ancestral Susceptible Genetic Pool
    Diancourt, Laure
    Passet, Virginie
    Nemec, Alexandr
    Dijkshoorn, Lenie
    Brisse, Sylvain
    [J]. PLOS ONE, 2010, 5 (03):
  • [24] The Rhizome of the Multidrug-Resistant Enterobacter aerogenes Genome Reveals How New "Killer Bugs" Are Created because of a Sympatric Lifestyle
    Diene, Seydina M.
    Merhej, Vicky
    Henry, Mireille
    El Filali, Adil
    Roux, Veronique
    Robert, Catherine
    Azza, Said
    Gavory, Frederick
    Barbe, Valerie
    La Scola, Bernard
    Raoult, Didier
    Rolain, Jean-Marc
    [J]. MOLECULAR BIOLOGY AND EVOLUTION, 2013, 30 (02) : 369 - 383
  • [25] First report of a NDM-producing Providencia rettgeri strain in the state of Sao Paulo
    do Carmo Junior, Nelson Vieira
    Ferreira Filho, Heli
    Gomes e Costa, Durval Alex
    Westheimer Calvalcante, Adilson Joaquim
    Garcia, Doroti de Oliveira
    Duailib Furtado, Juvencio Jose
    [J]. BRAZILIAN JOURNAL OF INFECTIOUS DISEASES, 2015, 19 (06) : 675 - 676
  • [26] Complete nucleotide sequence of the multidrug resistance IncA/C plasmid pR55 from Klebsiella pneumoniae isolated in 1969
    Doublet, Benoit
    Boyd, David
    Douard, Gregory
    Praud, Karine
    Cloeckaert, Axel
    Mulvey, Michael R.
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2012, 67 (10) : 2354 - 2360
  • [27] Outbreak of NDM-1-Producing Klebsiella pneumoniae in a Neonatal Unit in Colombia
    Escobar Perez, Javier Antonio
    Olarte Escobar, Narda Mara
    Castro-Cardozo, Betsy
    Valderrama Marquez, Ismael Alberto
    Garzon Aguilar, Martha Isabel
    Martinez de la Barrera, Leslie
    Barrero Barreto, Esther Rocio
    Alejandro Marquez-Ortiz, Ricaurte
    Moncada Guayazan, Maria Victoria
    Vanegas Gomez, Natasha
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2013, 57 (04) : 1957 - 1960
  • [28] Dissemination of an NDM-2-Producing Acinetobacter baumannii Clone in an Israeli Rehabilitation Center
    Espinal, P.
    Fugazza, G.
    Lopez, Y.
    Kasma, M.
    Lerman, Y.
    Malhotra-Kumar, S.
    Goossens, H.
    Carmeli, Y.
    Vila, J.
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2011, 55 (11) : 5396 - 5398
  • [29] Identification of NDM-1 in a Putatively Novel Acinetobacter Species ("NB14") Closely Related to Acinetobacter pittii
    Espinal, Paula
    Mosqueda, Noraida
    Telli, Murat
    van der Reijden, Tanny
    Rolo, Dora
    Fernandez-Orth, Dietmar
    Dijkshoorn, Lenie
    Roca, Ignasi
    Vila, Jordi
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2015, 59 (10) : 6657 - 6660
  • [30] Coexistence of a novel KPC-2-encoding MDR plasmid and an NDM-1-encoding pNDM-HN380-like plasmid in a clinical isolate of Citrobacter freundii
    Feng, Jiao
    Qiu, Yefeng
    Yin, Zhe
    Chen, Weijun
    Yang, Huiying
    Yang, Wenhui
    Wang, Jie
    Gao, Yingjie
    Zhou, Dongsheng
    [J]. JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2015, 70 (11) : 2987 - 2991