Enhancement of oxidative response and cytokine production by yam mucopolysaccharide in murine peritoneal macrophage

被引:23
作者
Choi, EM
Hwang, JK [1 ]
机构
[1] Yonsei Univ, Dept Biotechnol, Seoul 120749, South Korea
[2] Yonsei Univ, Bioprod Res Ctr, Seoul 120749, South Korea
关键词
yam mucopolysaccharide; macrophage; nitrite; H2O2; cytokine;
D O I
10.1016/S0367-326X(02)00238-1
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Yam (Dioscorea batatas) mucopolysaccharide (YMP) was examined for the ability to induce secretory and cellular responses in murine peritoneal macrophages. When macrophages were treated with various doses (10-100 mug/ml) of YMP for 48 h, a significant cytotoxicity against B 16 cells was observed. YMP also increased the myeloperoxidase activity and the production of H2O2, O-2(-), nitric oxide (NO) and tumor necrosis factor (TNF-alpha). However, there was a little alteration on the production of IFN-gamma. These results indicate that reactive oxygen intermediates (ROI), reactive nitrogen intermediates (RNI) and TNF-alpha are likely major mediators of cytotoxic activity of YMP-treated macrophages. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:629 / 637
页数:9
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