Sulfation of silk sericin and anticoagulant activity of sulfated sericin

被引：56

作者：

Tamada, Y

Sano, M

Niwa, K

Imai, T

Yoshino, G

机构：

[1] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058634, Japan

[2] Toho Univ, Fac Med, Sch Med, Dept Lab Med, Tokyo 1431234, Japan

[3] Toho Univ, Fac Sci, Dept Biol Hematol, Chiba 2748510, Japan

[4] Toho Univ, Fac Sci, Dept Physiol Chem, Chiba 2748510, Japan

来源：

JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | 2004年 / 15卷 / 08期

关键词：

sericin; sulfation; anticoagulant activity; gel-filtration chromatography;

D O I：

10.1163/1568562041526469

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Silk sericin extracted from a cocoon was sulfated by chlorosulfonic acid. Sulfation of sericin was confirmed by FT-IR and the reaction efficiency was calculated as 44.9%. H-1-NMR suggested that sulfation mainly occurred at serine residues in the sericin molecule. The sulfated sericin was separated in three fractions by gel-filtration chromatography using Sephacryl S-200. The sulfate group content and amino-acid composition of each fraction were almost identical, while the anticoagulant activity differed for each fraction of sulfated sericin. Higher anticoagulant activity was ;observed for the higher-molecular-mass fraction. The anticoagulant activity of sulfated sericin was estimated at 1/10 to 1/20 of heparin.

引用

页码：971 / 980

页数：10

共 21 条

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