Plasma prefractionation methods for proteomic analysis and perspectives in clinical applications

被引:17
作者
Chutipongtanate, Somchai [1 ]
Chatchen, Supawat [2 ]
Svasti, Jisnuson [3 ,4 ]
机构
[1] Mahidol Univ, Ramathibodi Hosp, Fac Med, Pediat Translat Res Unit,Dept Pediat, Salaya 10400, Nakhon Pathom, Thailand
[2] Mahidol Univ, Fac Trop Med, Dept Trop Pediat, Salaya, Nakhon Pathom, Thailand
[3] Chulabhorn Res Inst, Biochem Lab, Krung Thep Maha Nakhon, Thailand
[4] Chulabhorn Grad Inst, Appl Biol Sci Program, Bangkok, Thailand
关键词
Biomarker; Depletion; Enrichment; Fractionation; Plasma; INTRAUTERINE GROWTH RESTRICTION; LECTIN AFFINITY-CHROMATOGRAPHY; APOLIPOPROTEIN-A-I; PROTEIN-PHOSPHORYLATION PATTERNS; MASS-SPECTROMETRIC ANALYSIS; POTENTIAL SERUM BIOMARKER; MULTIVARIATE INDEX ASSAY; PATHOLOGISTS Q-PROBES; LIQUID-CHROMATOGRAPHY; CANDIDATE BIOMARKERS;
D O I
10.1002/prca.201600135
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Plasma is a rich source of biomarkers with clinical relevance. However, the wide dynamic range of protein concentration hinders the detection of low abundance proteins. Plasma prefractionation methods serve as indispensable tools to reduce plasma complexity, allowing the opportunity to explore tissue-derived proteins which leak into the circulation. This review summarizes common approaches in plasma prefractionation methods for proteomic analysis and then discusses some considerations in plasma prefractionation for clinical applications, reviewing some examples of its use in clinical situations.
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页数:13
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