NAC Candidate Gene Marker for bgm-1 and Interaction With QTL for Resistance to Bean Golden Yellow Mosaic Virus in Common Bean

被引:12
作者
Soler-Garzon, Alvaro [1 ]
Oladzad, Atena [2 ]
Beaver, James [3 ]
Beebe, Stephen [4 ]
Lee, Rian [2 ]
Lobaton, Juan David [4 ,5 ]
Macea, Eliana [4 ]
McClean, Phillip [2 ]
Raatz, Bodo [4 ]
Rosas, Juan Carlos [6 ]
Song, Qijian [7 ]
Miklas, Phillip N. [1 ,8 ]
机构
[1] Washington State Univ, Irrigated Agr Res & Extens Ctr, Prosser, WA 99350 USA
[2] North Dakota State Univ, Dept Plant Sci, Fargo, ND USA
[3] Univ Puerto Rico, Dept Agroenvironm Sci, Mayaguez, PR USA
[4] Int Ctr Trop Agr CIAT, Agrobiodivers Area, Bean Program, Cali, Colombia
[5] Univ New England, Sch Environm & Rural Sci, Armidale, SA, Australia
[6] Zamorano Univ, Dept Agr Engn, Zamorano, Honduras
[7] USDA ARS, Soybean Genom & Improvement Lab, Beltsville, MD USA
[8] USDA ARS, Grain Legume Genet & Physiol Res Unit, Prosser, WA 99350 USA
来源
FRONTIERS IN PLANT SCIENCE | 2021年 / 12卷
关键词
frameshift mutation; geminivirus; genome-wide association study; marker-assisted selection; Phaseolus vulgaris; DISEASE RESISTANCE; TRANSCRIPTION FACTORS; GENOME SEQUENCE; BCT GENE; ASSOCIATION; PROTEIN; GEMINIVIRUSES; REGISTRATION; INHERITANCE; GENERATION;
D O I
10.3389/fpls.2021.628443
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Genetic resistance is the primary means for control of Bean golden yellow mosaic virus (BGYMV) in common bean (Phaseolus vulgaris L.). Breeding for resistance is difficult because of sporadic and uneven infection across field nurseries. We sought to facilitate breeding for BGYMV resistance by improving marker-assisted selection (MAS) for the recessive bgm-1 gene and identifying and developing MAS for quantitative trait loci (QTL) conditioning resistance. Genetic linkage mapping in two recombinant inbred line populations and genome-wide association study (GWAS) in a large breeding population and two diversity panels revealed a candidate gene for bgm-1 and three QTL BGY4.1, BGY7.1, and BGY8.1 on independent chromosomes. A mutation (5 bp deletion) in a NAC (No Apical Meristem) domain transcriptional regulator superfamily protein gene Phvul.003G027100 on chromosome Pv03 corresponded with the recessive bgm-1 resistance allele. The five bp deletion in exon 2 starting at 20 bp (Pv03: 2,601,582) is expected to cause a stop codon at codon 23 (Pv03: 2,601,625), disrupting further translation of the gene. A T-m-shift assay marker named PvNAC1 was developed to track bgm-1. PvNAC1 corresponded with bgm-1 across similar to 1,000 lines which trace bgm-1 back to a single landrace "Garrapato" from Mexico. BGY8.1 has no effect on its own but exhibited a major effect when combined with bgm-1. BGY4.1 and BGY7.1 acted additively, and they enhanced the level of resistance when combined with bgm-1. T-m-shift assay markers were generated for MAS of the QTL, but their effectiveness requires further validation.
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页数:18
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