Refractive Index Determination of Nanoparticles in Suspension Using Nanoparticle Tracking Analysis

被引：160

作者：

van der Pol, Edwin ^{[1
,2
]}

Coumans, Frank A. W. ^{[1
,2
]}

Sturk, Auguste ^{[2
]}

Nieuwland, Rienk ^{[2
]}

van Leeuwen, Ton G. ^{[1
]}

机构：

[1] Univ Amsterdam, Acad Med Ctr, NL-1100 DD Amsterdam, Netherlands

[2] Univ Amsterdam, Acad Med Ctr, Lab Expt Clin Chem, NL-1100 DD Amsterdam, Netherlands

来源：

NANO LETTERS | 2014年 / 14卷 / 11期

关键词：

Drug delivery; exosomes; extracellular vesicles; microparticles; nanomedicine; nanoparticles; GOLD NANORODS; CELLS; SIZE; MICROPARTICLES; VESICLES; MICROSPHERES; PARTICLES; PROTEINS; EXOSOMES; CARBON;

D O I：

10.1021/nl503371p

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The refractive index (RI) dictates interaction between light and nanoparticles and therefore is important to health, environmental, and materials sciences. Using nanoparticle tracking analysis, we have determined the RI of heterogeneous particles <500 nm in suspension. We demonstrate feasibility of distinguishing silica and polystyrene beads based on their RI. The hitherto unknown RI of extracellular vesicles from human urine was determined at 1.37 (mean). This method enables differentiation of single nanoparticles based on their RI.

引用

页码：6195 / 6201

页数：7

共 59 条

[1] SIZE AND REFRACTIVE-INDEX OF INDIVIDUAL MARINE PARTICULATES - A FLOW CYTOMETRIC APPROACH [J].

ACKLESON, SG ;

SPINRAD, RW .

APPLIED OPTICS, 1988, 27 (07) :1270-1277

[2] Behaviour of nanoparticle (ultrafine) titanium dioxide pigments and stabilisers on the photooxidative stability of water based acrylic and isocyanate based acrylic coatings [J].

Allen, NS ;

Edge, M ;

Ortega, A ;

Liauw, CM ;

Stratton, J ;

McIntyre, RB .

POLYMER DEGRADATION AND STABILITY, 2002, 78 (03) :467-478

[3]

[Anonymous], 2008, MATL PART TRACK COD

[4] REFRACTOMETRY AND INTERFEROMETRY OF LIVING CELLS [J].

BARER, R .

JOURNAL OF THE OPTICAL SOCIETY OF AMERICA, 1957, 47 (06) :545-556

[5] REFRACTIVE INDEX OF CONCENTRATED PROTEIN SOLUTIONS [J].

BARER, R ;

TKACZYK, S .

NATURE, 1954, 173 (4409) :821-822

[6] CCD based approach to high-precision size and refractive index determination of levitated microdroplets using Fraunhofer diffraction [J].

Barnes, MD ;

Lermer, N ;

Whitten, WB ;

Ramsey, JM .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1997, 68 (06) :2287-2291

[7] Expanding the Optical Trapping Range of Lipid Vesicles to the Nanoscale [J].

Bendix, Poul M. ;

Oddershede, Lene B. .

NANO LETTERS, 2011, 11 (12) :5431-5437

[8] The spatial variation of the refractive index in biological cells [J].

Beuthan, J ;

Minet, O ;

Helfmann, J ;

Herrig, M ;

Muller, G .

PHYSICS IN MEDICINE AND BIOLOGY, 1996, 41 (03) :369-382

[9] Bacterial Vesicles in Marine Ecosystems [J].

Biller, Steven J. ;

Schubotz, Florence ;

Roggensack, Sara E. ;

Thompson, Anne W. ;

Summons, Roger E. ;

Chisholm, Sallie W. .

SCIENCE, 2014, 343 (6167) :183-186

[10]

Bohren C.F, 2008, Absorption and Scattering of Light by Small Particles

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