Targeting human urinary metabolome by LC-MS/MS: a review

被引：45

作者：

Rodriguez-Morato, Jose ^{[1
,2
,3
]}

Pozo, Oscar J. ^{[2
]}

Marcos, Josep ^{[1
,4
]}

机构：

[1] Univ Pompeu Fabra, CEXS, Dept Expt & Hlth Sci, Doctor Aiguader 88, Barcelona 08003, Spain

[2] Hosp Mar, IMIM, Integrat Pharmacol & Syst Neurosci, Barcelona 08003, Spain

[3] CIBEROBN, CIBER Fisiopatol Obesidad & Nutr, CB06-03-028, Santiago De Compostela 15706, Spain

[4] Cerba Int, Pl Ramon Llull 7, Sabadell 08203, Spain

来源：

BIOANALYSIS | 2018年 / 10卷 / 07期

基金：

欧盟地平线“2020”;

关键词：

amino acids; LC-MS/MS; lipids; neurotransmitters; quantitation; steroids; targeted metabolomics; untargeted metabolomics; urine; CHROMATOGRAPHY-MASS-SPECTROMETRY; PERFORMANCE LIQUID-CHROMATOGRAPHY; ATMOSPHERIC-PRESSURE PHOTOIONIZATION; UNDERIVATIZED AMINO-ACIDS; ENZYME-ASSISTED SYNTHESIS; SOLID-PHASE EXTRACTION; DOPING-CONTROL; ANABOLIC-STEROIDS; HUMAN PLASMA; BILE-ACIDS;

D O I：

10.4155/bio-2017-0285

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Urine is a biologicalmatrix that contains hundreds of metabolic end products which constitute the urinary metabolome. The development and advances on LC-MS/MS have revolutionized the analytical study of biomolecules by enabling their accurate identification and quantification in an unprecedented manner. Nowadays, LC-MS/MS is helping to unveil the complexity of urine metabolome, and the results obtained have multiple biomedical applications. This review focuses on the targeted LC-MS/MS analysis of the urine metabolome. In the first part, we describe general considerations (from sample collection to quantitation) required for a proper targeted metabolic analysis. In the second part, we address the urinary analysis and recent applications of four relevant families: amino acids, catecholamines, lipids and steroids.

引用

页码：489 / 516

页数：28

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