Retinoic acid depletion induces keratinizing squamous differentiation in human middle ear epithelial cell cultures

被引:13
作者
Choi, JY
Cho, KN
Yoo, KH
Shin, JH
Yoon, JH
机构
[1] Yonsei Univ, Coll Med, Dept Otorhinolaryngol, Seoul, South Korea
[2] Yonsei Univ, Coll Med, Project Med Sci BK21, Seoul, South Korea
关键词
cell culture; cholesteatoma; middle ear; retinoic acid; squamous differentiation;
D O I
10.1080/0036554021000028097
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
Objective-The pathogenesis of cholesteatoma behind an intact tympanic membrane remains controversial. Squamous metaplasia of the middle ear mucosa is thought to be a possible mechanism in such cases. However, to date, no definitive experimental results have proved this association. This study was undertaken to investigate whether normal human middle ear epithelial (NHMEE) cells undergo keratinizing squamous differentiation in a retinoic acid (RA)-deficient culture. Material and Methods-We examined the morphological differences between RA-deficient and -sufficient cultures, and determined the expressions of the mucin gene and cornifin-alpha mRNAs as indicators of mucous and squamous differentiation, respectively. Results-Histomorphologically, the NHMEE cells differentiated into a keratinizing squamous epithelium in RA-deficient cultures. In addition, the expressions of mucin gene 5AC (MUC5AC) and MUC8 mRNAs were suppressed, and the expression of cornifin-alpha mRNA increased progressively as a function of differentiation in RA-deficient cultures. Conclusions-This study shows that RA depletion induces keratinizing squamous differentiation in NHMEE cell cultures. This finding supports the hypothesis that middle ear cholesteatoma originates from metaplastic middle ear mucosa.
引用
收藏
页码:466 / 470
页数:5
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