Detection of Non-Amplified Mycobacterium tuberculosis Genomic DNA Using Piezoelectric DNA-Based Biosensors

被引：30

作者：

Kaewphinit, Thongchai ^{[1
]}

Santiwatanakul, Somchai ^{[2
]}

Promptmas, Chamras ^{[3
]}

Chansiri, Kosum ^{[1
]}

机构：

[1] Srinakharinwirot Univ, Fac Med, Dept Biochem, Bangkok 10110, Thailand

[2] Srinakharinwirot Univ, Fac Med, Dept Pathol, Bangkok 10110, Thailand

[3] Mahidol Univ, Fac Med Technol, Dept Clin Chem, Bangkok 10700, Thailand

来源：

SENSORS | 2010年 / 10卷 / 03期

关键词：

Mycobacterium tuberculosis; piezoelectric biosensor; non-amplified genomic DNA; POLYMERASE-CHAIN-REACTION; IS6110; MICROBALANCE; SEQUENCES; DIAGNOSIS; PULMONARY; ASSAY; TIME;

D O I：

10.3390/s100301846

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Piezoelectric DNA-based biosensor technology was developed as a new method for detection of M. tuberculosis. This method consists of immobilizing a thiol-modified oligonucleotide probe on the gold electrode surface of a quartz crystal, using a self-assembled monolayer method. The advantage of this study is that a non-amplified genomic bacterial DNA target was used. Instead, the genomic DNA was digested by restriction enzyme to obtain DNA fragments containing the target sequence. The fabricated biosensor was evaluated through an examination of 200 samples. No cross hybridization were observed against M. avium complex and other microorganisms. This target DNA preparation, without PCR amplification, will reduce time, costs, and the tedious step of amplification.

引用

页码：1846 / 1858

页数：13

共 22 条

[1] Variable-number-tandem repeat typing of mycobacterium tuberculosis isolates with low copy numbers of IS6110 by using mycobacterial interspersed repetitive units [J].