Multispectral Imaging of T and B Cells in Murine Spleen and Tumor

被引：58

作者：

Feng, Zipei ^{[1
,2
]}

Jensen, Shawn M. ^{[1
]}

Messenheimer, David J. ^{[1
,3
]}

Farhad, Mohammed ^{[1
,2
]}

Neuberger, Michael ^{[1
,5
]}

Bifulco, Carlo B. ^{[4
]}

Fox, Bernard A. ^{[1
,3
]}

机构：

[1] Providence Portland Med Ctr, Earle A Chiles Res Inst, Providence Canc Ctr, Robert W Franz Canc Res Ctr, Portland, OR 97213 USA

[2] Oregon Hlth & Sci Univ, Dept Cell Dev & Canc Biol, Portland, OR 97239 USA

[3] Oregon Hlth & Sci Univ, Dept Mol Microbiol & Immunol, Portland, OR 97239 USA

[4] Providence Portland Med Ctr, Dept Pathol, Portland, OR 97213 USA

[5] Hosp Ludwig Maximilian Univ, Dept Gen Visceral Transplantat Vasc & Thorac Surg, Munich, Germany

来源：

JOURNAL OF IMMUNOLOGY | 2016年 / 196卷 / 09期

关键词：

SURFACE-ANTIGENS; HUMAN-TISSUES; IMMUNE CELLS; LUNG-CANCER; IMMUNOHISTOCHEMISTRY; PEMBROLIZUMAB; PRESERVATION; MACROPHAGES; FIXATION; PROTEINS;

D O I：

10.4049/jimmunol.1502635

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Recent advances in multiplex immunohistochemistry techniques allow for quantitative, spatial identification of multiple immune parameters for enhanced diagnostic and prognostic insight. However, applying such techniques to murine fixed tissues, particularly sensitive epitopes, such as CD4, CD8 alpha, and CD19, has been difficult. We compared different fixation protocols and Ag-retrieval techniques and validated the use of multiplex immunohistochemistry for detection of CD3+CD4+ and CD3+CD8+ T cell subsets in murine spleen and tumor. This allows for enumeration of these T cell subsets within immune environments, as well as the study of their spatial distribution.

引用

收藏

页码：3943 / 3950

页数：8

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