Induction of Phlorotannins and Gene Expression in the Brown Macroalga Fucus vesiculosus in Response to the Herbivore Littorina littorea

被引:10
|
作者
Creis, Bendelac Emeline [1 ,2 ]
Delage, Ludovic [1 ]
Vallet, Laurent [1 ]
Leblanc, Catherine [1 ]
Inken, Kruse [3 ]
Ar, Gall Erwan [4 ]
Weinberger, Florian [3 ]
Potin, Philippe [1 ]
机构
[1] Sorbonne Univ, Integrat Biol Marine Models LBI2M, Stn Biol, CNRS,UMR 8227, Pl Georges Teissier, F-29680 Roscoff, France
[2] Sorbonne Univ, Int Res Lab IRL 3614, CNRS,Evolutionary Biol & Ecol Algae,EBEA, Stn Biol,PUC,UACH, F-29680 Roscoff, France
[3] Helmholtz Ctr Ocean Res GEOMAR, Dusternbrooker Weg 20, D-24105 Kiel, Germany
[4] Univ Brest Western Brittany, UBO European Inst Marine Studies IUEM, Lab Sci Environm Marin, IUEM,UMR 6539 LEMAR,Technopole Brest Iroise, Rue Dumt Urville, F-29280 Plouzane, France
关键词
phlorotannins; inducible defense; gene expression; grazing; Fucus vesiculosus; ECKLONIA-STOLONIFERA; PHENOLIC SUBSTANCES; BIOTIC INTERACTIONS; INDUCED RESISTANCE; ALGAE; POLYPHENOLICS; INSIGHTS; WALL;
D O I
10.3390/md19040185
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
Mechanisms related to the induction of phlorotannin biosynthesis in marine brown algae remain poorly known. Several studies undertaken on fucoid species have shown that phlorotannins accumulate in the algae for several days or weeks after being exposed to grazing, and this is measured by direct quantification of soluble phenolic compounds. In order to investigate earlier inducible responses involved in phlorotannin metabolism, Fucus vesiculosus was studied between 6 and 72 h of grazing by the sea snail Littorina littorea. In this study, the quantification of soluble phenolic compounds was complemented by a Quantitative real-time PCR (qRT-PCR) approach applied on genes that are potentially involved in either the phlorotannin metabolism or stress responses. Soluble phlorotannin levels remained stable during the kinetics and increased significantly only after 12 h in the presence of grazers, compared to the control, before decreasing to the initial steady state for the rest of the kinetics. Under grazing conditions, the expression of vbpo, cyp450 and ast6 genes was upregulated, respectively, at 6 h, 12 h and 24 h, and cyp450 gene was downregulated after 72 h. Interestingly, the pksIII gene involved in the synthesis of phloroglucinol was overexpressed under grazing conditions after 24 h and 72 h. This study supports the hypothesis that phlorotannins are able to provide an inducible chemical defense under grazing activity, which is regulated at different stages of the stress response.
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页数:15
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