A Graphene Nanoprobe for Rapid, Sensitive, and Multicolor Fluorescent DNA Analysis

被引:1277
作者
He, Shijiang [1 ]
Song, Bo [1 ]
Li, Di [1 ]
Zhu, Changfeng [1 ]
Qi, Wenpeng [1 ]
Wen, Yanqin [1 ]
Wang, Lihua [1 ]
Song, Shiping [1 ]
Fang, Haiping [1 ]
Fan, Chunhai [1 ]
机构
[1] Chinese Acad Sci, Shanghai Inst Appl Phys, Phys Biol Lab, Shanghai 201800, Peoples R China
关键词
COLORIMETRIC DETECTION; CONJUGATED POLYMERS; MOLECULAR BEACONS; PI-STACKING; IN-VITRO; PROBES; MERCURY(II); SYSTEMS; SENSOR; OXIDE;
D O I
10.1002/adfm.200901639
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Coupling nanomaterials with biomolecular recognition events represents a new direction in nanotechnology toward the development of novel molecular diagnostic tools. Here a graphene oxide (GO)-based multicolor fluorescent DNA nanoprobe that allows rapid, sensitive, and selective detection of DNA targets in homogeneous solution by exploiting interactions between GO and DNA molecules is reported. Because of the extraordinarily high quenching efficiency of GO, the fluorescent ssDNA probe exhibits minimal background fluorescence, while strong emission is observed when it forms a double helix with the specific targets, leading to a high signal-to-background ratio. Importantly, the large planar surface of GO allows simultaneous quenching of multiple DNA probes labeled with different dyes, leading to a multicolor sensor for the detection of multiple DNA targets in the same solution. It is also demonstrated that this GO-based sensing platform is suitable for the detection of a range of analytes when complemented with the use of functional DNA structures.
引用
收藏
页码:453 / 459
页数:7
相关论文
共 51 条
[1]   Near-infrared optical sensors based on single-walled carbon nanotubes [J].
Barone, PW ;
Baik, S ;
Heller, DA ;
Strano, MS .
NATURE MATERIALS, 2005, 4 (01) :86-U16
[2]   PI-STACKING AND AGGREGATION OF PYRIDINIUM-SUBSTITUTED INDOLIZINES [J].
CARTER, PW ;
DIMAGNO, SG ;
PORTER, JD ;
STREITWIESER, A .
JOURNAL OF PHYSICAL CHEMISTRY, 1993, 97 (05) :1085-1096
[3]   PARTICLE MESH EWALD - AN N.LOG(N) METHOD FOR EWALD SUMS IN LARGE SYSTEMS [J].
DARDEN, T ;
YORK, D ;
PEDERSEN, L .
JOURNAL OF CHEMICAL PHYSICS, 1993, 98 (12) :10089-10092
[4]   Preparation and characterization of graphene oxide paper [J].
Dikin, Dmitriy A. ;
Stankovich, Sasha ;
Zimney, Eric J. ;
Piner, Richard D. ;
Dommett, Geoffrey H. B. ;
Evmenenko, Guennadi ;
Nguyen, SonBinh T. ;
Ruoff, Rodney S. .
NATURE, 2007, 448 (7152) :457-460
[5]   Symmetry Breaking of Graphene Monolayers by Molecular Decoration [J].
Dong, Xiaochen ;
Shi, Yumeng ;
Zhao, Yang ;
Chen, Dongmeng ;
Ye, Jun ;
Yao, Yugui ;
Gao, Fang ;
Ni, Zhenhua ;
Yu, Ting ;
Shen, Zexiang ;
Huang, Yinxi ;
Chen, Peng ;
Li, Lain-Jong .
PHYSICAL REVIEW LETTERS, 2009, 102 (13)
[6]   Single-mismatch detection using gold-quenched fluorescent oligonucleotides [J].
Dubertret, B ;
Calame, M ;
Libchaber, AJ .
NATURE BIOTECHNOLOGY, 2001, 19 (04) :365-370
[7]   Selective colorimetric detection of polynucleotides based on the distance-dependent optical properties of gold nanoparticles [J].
Elghanian, R ;
Storhoff, JJ ;
Mucic, RC ;
Letsinger, RL ;
Mirkin, CA .
SCIENCE, 1997, 277 (5329) :1078-1081
[8]   INVITRO SELECTION OF RNA MOLECULES THAT BIND SPECIFIC LIGANDS [J].
ELLINGTON, AD ;
SZOSTAK, JW .
NATURE, 1990, 346 (6287) :818-822
[9]   Beyond superquenching: Hyper-efficient energy transfer from conjugated polymers to gold nanoparticles [J].
Fan, CH ;
Wang, S ;
Hong, JW ;
Bazan, GC ;
Plaxco, KW ;
Heeger, AJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (11) :6297-6301
[10]   Rational design of DNA nanoarchitectures [J].
Feldkamp, U ;
Niemeyer, CM .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (12) :1856-1876