Adaptation of FUN-1 and Calcofluor white stains to assess the ability of viable and nonviable yeast to adhere to and be internalized by cultured mammalian cells

被引：33

作者：

Henry-Stanley, MJ ^{[1
]}

Garni, RM ^{[1
]}

Wells, CL ^{[1
]}

机构：

[1] Univ Minnesota, Dept Lab Med & Pathol, Minneapolis, MN 55455 USA

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2004年 / 59卷 / 02期

关键词：

yeast; FUN-1; calcofluor white; viability; intracellular;

D O I：

10.1016/j.mimet.2004.07.001

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The FUN-1 and Calcofluor white stains can be used in concert to assess the ability of viable and nonviable yeast to adhere to, and be internalized by, host mammalian cells in vitro. With this method, only extracellular yeast stain with Calcofluor, dead yeast cells have diffuse cytoplasmic yellow-green fluorescence, and live yeast have cytoplasmic orange-red or yellow-orange fluorescent intravacuolar structures. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：289 / 292

页数：4

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