Distribution of the verrucomicrobial clade Spartobacteria along a salinity gradient in the Baltic Sea

被引：29

作者：

Bergen, Benjamin ^{[1
]}

Herlemann, Daniel P. R. ^{[1
]}

Labrenz, Matthias ^{[1
]}

Juergens, Klaus ^{[1
]}

机构：

[1] Leibniz Inst Balt Sea Res Warnemnde IOW, D-18119 Rostock, Germany

来源：

ENVIRONMENTAL MICROBIOLOGY REPORTS | 2014年 / 6卷 / 06期

关键词：

IN-SITU HYBRIDIZATION; 16S RIBOSOMAL-RNA; BACTERIOPLANKTON COMMUNITY; DIVERSITY; SEQUENCE; IDENTIFICATION; BACTERIA;

D O I：

10.1111/1758-2229.12178

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A recent pyrosequencing study along the whole Baltic Sea salinity transect identified members of the Verrucomicrobia class Spartobacteria as an important component of Baltic Sea bacterioplankton. In this study, catalysed reporter deposition-fluorescence in situ hybridization was used for cellular quantification. The published probes VER47 and SPA714 were optimized for samples from the Baltic Sea and a new, specific probe (SPA476) was used to quantify the dominant spartobacterial lineage LD29'. The results confirmed that in the brackish surface waters of the Baltic Sea Spartobacteria comprise an important component, constituting up to 12% of all bacteria. The positive correlation and physical association of Spartobacteria with phytoplankton suggest their involvement in the utilization of phytoplankton-derived organic matter in the Baltic Sea.

引用

页码：625 / 630

页数：6

共 21 条

[1] Single-cell identification in microbial communities by improved fluorescence in situ hybridization techniques [J].

Amann, Rudolf ;

Fuchs, Bernhard M. .

NATURE REVIEWS MICROBIOLOGY, 2008, 6 (05) :339-348

[2] Pyrosequencing reveals contrasting seasonal dynamics of taxa within Baltic Sea bacterioplankton communities [J].

Andersson, Anders F. ;

Riemann, Lasse ;

Bertilsson, Stefan .

ISME JOURNAL, 2010, 4 (02) :171-181

[3] Development of a 16S rRNA-targeted probe set for Verrucomicrobia and its application for fluorescence in situ hybridization in a humic lake [J].

Arnds, Julia ;

Knittel, Katrin ;

Buck, Ulrike ;

Winkel, Matthias ;

Amann, Rudolf .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 2010, 33 (03) :139-148

[4] Environmental factors influencing the distribution of rRNA from Verrucomicrobia in soil [J].

Buckley, DH ;

Schmidt, TM .

FEMS MICROBIOLOGY ECOLOGY, 2001, 35 (01) :105-112

[5] The domain-specific probe EUB338 is insufficient for the detection of all Bacteria:: Development and evaluation of a more comprehensive probe set [J].

Daims, H ;

Brühl, A ;

Amann, R ;

Schleifer, KH ;

Wagner, M .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1999, 22 (03) :434-444

[6] Global distribution and diversity of marine Verrucomicrobia [J].

Freitas, Sara ;

Hatosy, Stephen ;

Fuhrman, Jed A. ;

Huse, Susan M. ;

Welch, David B. Mark ;

Sogin, Mitchell L. ;

Martiny, Adam C. .

ISME JOURNAL, 2012, 6 (08) :1499-1505

[7] Unlabeled helper oligonucleotides increase the in situ accessibility to 16S rRNA of fluorescently labeled oligonucleotide probes [J].

Fuchs, BM ;

Glöckner, FO ;

Wulf, J ;

Amann, R .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2000, 66 (08) :3603-3607

[8] Metagenomic De Novo Assembly of an Aquatic Representative of the Verrucomicrobial Class Spartobacteria [J].

Herlemann, Daniel P. R. ;

Lundin, Daniel ;

Labrenz, Matthias ;

Juergens, Klaus ;

Zheng, Zongli ;

Aspeborg, Henrik ;

Andersson, Anders F. .

MBIO, 2013, 4 (03)

[9] Transitions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea [J].

Herlemann, Daniel P. R. ;

Labrenz, Matthias ;

Juergens, Klaus ;

Bertilsson, Stefan ;

Waniek, Joanna J. ;

Andersson, Anders F. .

ISME JOURNAL, 2011, 5 (10) :1571-1579

[10] Bacterioplankton community in Chesapeake Bay: Predictable or random assemblages [J].

Kan, Jinjun ;

Crump, Byron C. ;

Wang, Kui ;

Chen, Feng .

LIMNOLOGY AND OCEANOGRAPHY, 2006, 51 (05) :2157-2169

← 1 2 3 →