Legionella septentrionalis sp. nov., isolated from aquatic environments in the northern PR China

Four strains (km711(T), km714, km542 and km524), representing a novel Legionella species, were isolated from aquatic environments in northern PR China. Cells were Gram-stain-negative, rod-shaped, microaerobic, motile and growth depended on l-cysteine. They grew at 25.42 degrees C (optimum, 35.37 degrees C) and could tolerate up to 1.5% (w/v) NaCl (optimum, 0.5%). The major fatty acids (>5 %) of the type strain km711(T) were C-17:0 anteiso, C-15:0 anteiso, iso-C-16:0 and C-16:1 omega 7c and/or iso-C-15:0 2OH. The pairwise comparison values were <96.1% for 16S rRNA gene sequences, 23.3.28.7 % interspecies variation for mip gene sequences, <93.6% average nucleotide identity and <72.8 % average amino acid identity between these four strains and related type strains within the genus Legionella. The phylogenetic tree based on the four concatenated genes (16S rRNA, mip, rpoB and rnpB) and protein-concatamer tree based on concatenation of 21 protein markers both revealed that these four strains formed a separate phylogenetic branch cluster within the genus Legionella. The results of phenotypic and genotypic features suggest that these four strains represent a novel species of the genus Legionella, for which the name Legionella septentrionalis sp. nov. is proposed (type strain km711(T)=KCTC 15655(T)=NBRC 113219(T)).