SARS-CoV-2 spike variants exhibit differential infectivity and neutralization resistance to convalescent or post-vaccination sera

被引：148

作者：

Kuzmina, Alona ^{[1
]}

Khalaila, Yara ^{[2
]}

Voloshin, Olga ^{[2
]}

Keren-Naus, Ayelet ^{[2
]}

Boehm-Cohen, Liora ^{[2
]}

Raviv, Yael ^{[2
]}

Shemer-Avni, Yonat ^{[1
,2
]}

Rosenberg, Elli ^{[2
]}

Taube, Ran ^{[1
]}

机构：

[1] Ben Gurion Univ Negev, Fac Hlth Sci, Shraga Segal Dept Microbiol Immunol & Genet, Beer Sheva, Israel

[2] Soroka Med Ctr, Beer Sheva, Israel

来源：

CELL HOST & MICROBE | 2021年 / 29卷 / 04期

基金：

以色列科学基金会;

关键词：

RECEPTOR-BINDING DOMAIN; TRANSMISSION;

D O I：

10.1016/j.chom.2021.03.008

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Toward eradicating the COVID-19 pandemic, vaccines that induce high humoral and cellular immune responses are essential. However, SARS-CoV-2 variants have begun to emerge and raise concerns, as they may potentially compromise vaccine efficiency. Here, we monitored neutralization potency of convalescent or Pfizer-BTN162b2 post-vaccination sera against pseudoviruses displaying spike proteins derived from wild-type SARS-CoV-2, or its UK-B.1.1.7 and SA-B.1.351 variants. Compared to convalescent sera, vaccination induces high titers of neutralizing antibodies, which exhibit efficient neutralization potential against pseudovirus carrying wild-type SARS-CoV-2. However, while wild-type and UK-N501Y pseudoviruses were similarly neutralized, those displaying SA-N501Y/K417N/E484K spike mutations moderately resist neutralization. Contribution of single or combined spike mutations to neutralization and infectivity were monitored, highlighting mechanisms by which viral infectivity and neutralization resistance are enhanced by N501Y or E484K/K417N mutations. Our study validates the importance of the Pfizer vaccine but raises concerns regarding its efficacy against specific SARS-CoV-2 circulating variants.

引用

页码：522 / +

页数：9

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