Genotoxic and cell-transforming effects of titanium dioxide nanoparticles

被引：52

作者：

Demir, Esref ^{[1
,5
]}

Akca, Hakan ^{[2
]}

Turna, Fatma ^{[1
]}

Aksakal, Sezgin ^{[1
]}

Burgucu, Durmus ^{[3
,4
]}

Kaya, Bulent ^{[1
]}

Tokgun, Onur ^{[2
]}

Vales, Gerard ^{[5
]}

Creus, Amadeu ^{[5
,6
]}

Marcos, Ricard ^{[5
,6
]}

机构：

[1] Akdeniz Univ, Fac Sci, Dept Biol, TR-07058 Antalya, Turkey

[2] Pamukkale Univ, Sch Med, Dept Med Biol, Kin, Denizli, Turkey

[3] Antalya Technopk Babylife Cord Blood Bank, TR-07058 Antalya, Turkey

[4] Stem Cell Res Ctr, TR-07058 Antalya, Turkey

[5] Univ Autonoma Barcelona, Fac Biociencies, Dept Genet & Microbiol, Grp Mutagenesi, Cerdanyola Del Valles 08193, Spain

[6] ISCIII, CIDER Epidemiol & Salud Publ, Madrid, Spain

来源：

ENVIRONMENTAL RESEARCH | 2015年 / 136卷

关键词：

Nano-TiO2; Comet assay; Micronucleus assay; Soft-agar assay; HEK293 and NIH/3T3 cells; DNA-DAMAGE; TIO2; NANOPARTICLES; OXIDATIVE STRESS; IN-VITRO; INDUCED CYTOTOXICITY; HUMAN-LYMPHOCYTES; CARBON NANOTUBES; COMET ASSAY; PARTICLES; KERATINOCYTES;

D O I：

10.1016/j.envres.2014.10.032

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The in vitro genotoxic and the soft-agar anchorage independent cell transformation ability of titanium dioxide nanoparticles (nano-TiO2) and its microparticulated form has been evaluated in human embryonic kidney (HEK293) and in mouse embryonic fibroblast (NIH/3T3) cells. Nano-TiO2 of two different sizes (21 and 50 nm) were used in this study. The comet assay, with and without the use of FPG enzyme, the micronucleus assay and the soft-agar colony assay were used. For both the comet assay and the frequency of micronuclei a statistically significant induction of DNA damage, was observed at the highest dose tested (1000 mu g/mL). No oxidative DNA damage induction was observed when the comet assay was complemented with the use of FPG enzyme. Furthermore, long-term exposure to nano-TiO2 has also proved to induce cell-transformation promoting cell-anchorage independent growth in soft-agar. Results were similar for the two nano-TiO2 sizes. Negative results were obtained when the microparticulated form of TiO2 was tested, indicating the existence of important differences between the microparticulated and nanoparticulated forms. As a conclusion it should be indicated that the observed genotoxic/tranforming effects were only detected at the higher dose tested (1000 mu g/mL) what play down the real risk of environmental exposures to this nanomaterial. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：300 / 308

页数：9

共 42 条

[21] Titanium dioxide nanoparticles exhibit genotoxicity and impair DNA repair activity in A549 cells [J].

Jugan, Mary-Line ;

Barillet, Sabrina ;

Simon-Deckers, Angelique ;

Herlin-Boime, Nathalie ;

Sauvaigo, Sylvie ;

Douki, Thierry ;

Carriere, Marie .

NANOTOXICOLOGY, 2012, 6 (05) :501-513

[22] Toxicological Aspects of Long-Term Treatment of Keratinocytes with ZnO and TiO2 Nanoparticles [J].

Kocbek, Petra ;

Teskac, Karmen ;

Kreft, Mateja E. ;

Kristl, Julijana .

SMALL, 2010, 6 (17) :1908-1917

[23]

Osman IF, 2010, NANOMEDICINE-UK, V5, P1193, DOI [10.2217/nnm.10.52, 10.2217/NNM.10.52]

[24] DNA damage and alterations in expression of DNA damage responsive genes induced by TiO2 nanoparticles in human hepatoma HepG2 cells [J].

Petkovic, Jana ;

Zegura, Bojana ;

Stevanovic, Magdalena ;

Drnovsek, Natasa ;

Uskokovic, Dragan ;

Novak, Sasa ;

Filipic, Metka .

NANOTOXICOLOGY, 2011, 5 (03) :341-353

[25]

Pojano G, 2010, NANOMATERIAL DISPERS

[26] Morphological transformation induced by multiwall carbon nanotubes on Balb/3T3 cell model as an in vitro end point of carcinogenic potential [J].

Ponti, Jessica ;

Broggi, Francesca ;

Mariani, Valentina ;

De Marzi, Laura ;

Colognato, Renato ;

Marmorato, Patrick ;

Gioria, Sabrina ;

Gilliland, Douglas ;

Garcia, Cesar Pascual ;

Meschini, Stefania ;

Stringaro, Annarita ;

Molinari, Agnese ;

Rauscher, Hubert ;

Rossi, Francois .

NANOTOXICOLOGY, 2013, 7 (02) :221-233

[27] Effect of Treatment Media on the Agglomeration of Titanium Dioxide Nanoparticles: Impact on Genotoxicity, Cellular Interaction, and Cell Cycle [J].

Prasad, Raju Y. ;

Wallace, Kathleen ;

Daniel, Kaitlin M. ;

Tennant, Alan H. ;

Zucker, Robert M. ;

Strickland, Jenna ;

Dreher, Kevin ;

Kligerman, Andrew D. ;

Blackman, Carl F. ;

DeMarini, David M. .

ACS NANO, 2013, 7 (03) :1929-1942

[28] Evidence that ultrafine titanium dioxide induces micronuclei and apoptosis in Syrian hamster embryo fibroblasts [J].

Rahman, Q ;

Lohani, M ;

Dopp, E ;

Pemsel, H ;

Jonas, L ;

Weiss, DG ;

Schiffmann, D .

ENVIRONMENTAL HEALTH PERSPECTIVES, 2002, 110 (08) :797-800

[29] Hydroxyl radicals (•OH) are associated with titanium dioxide (TiO2) nanoparticle-induced cytotoxicity and oxidative DNA damage in fish cells [J].

Reeves, James F. ;

Davies, Simon J. ;

Dodd, Nicholas J. F. ;

Jha, Awadhesh N. .

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 2008, 640 (1-2) :113-122

[30] Genotoxicity of TiO2 anatase nanoparticles in B6C3F1 male mice evaluated using Pig-a and flow cytometric micronucleus assays [J].

Sadiq, Rakhshinda ;

Bhalli, Javed A. ;

Yan, Jian ;

Woodruff, Robert S. ;

Pearce, Mason G. ;

Li, Yan ;

Mustafa, Thikra ;

Watanabe, Fumiya ;

Pack, Lindsay M. ;

Biris, Alexandru S. ;

Khan, Qaiser M. ;

Chen, Tao .

MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 2012, 745 (1-2) :65-72

← 1 2 3 4 5 →