Simultaneous two-plane, two-photon imaging based on spatial multiplexing

被引：4
作者：
Hu, Qinglei [1 ,2 ]
Li, Pei [1 ,2 ]
Xiong, Yumiao [1 ,2 ]
Wang, Yu [1 ,2 ]
Lv, Xiaohua [1 ,2 ]
Zeng, Shaoqun [1 ,2 ]
机构：
[1] Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelect, Britton Chance Ctr Biomed Photon, Wuhan 430074, Hubei, Peoples R China
[2] Huazhong Univ Sci & Technol, Sch Engn Sci, Collaborat Innovat Ctr Biomed Engn, MOE Key Lab Biomed Photon, Wuhan 430074, Hubei, Peoples R China
来源：
OPTICS LETTERS | 2018年 / 43卷 / 19期
基金：
中国国家自然科学基金;
关键词：
NEURONAL-ACTIVITY; MICROSCOPY; LENS; BRAIN;
D O I：
10.1364/OL.43.004598
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
The two-photon microscope is a powerful tool in life science. Conventional two-photon microscopy can image only a plane of a particular axial position at a time. Axial scanning can get the volumetric information, but it gets signals from different axial positions serially, which means that the exposure time at every plane is limited. Here we demonstrate a novel method, to the best of our knowledge, that can simultaneously record images from two planes at different xyz positions. The demultiplexing of the signal is realized using a confocal strategy. The experimental results show that it can be used for simultaneous two-photon imaging at two focal planes with little cross talk. (C) 2018 Optical Society of America
引用
收藏
页码：4598 / 4601
页数：4
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