Simultaneous two-plane, two-photon imaging based on spatial multiplexing

被引：4

作者：

Hu, Qinglei ^{[1
,2
]}

Li, Pei ^{[1
,2
]}

Xiong, Yumiao ^{[1
,2
]}

Wang, Yu ^{[1
,2
]}

Lv, Xiaohua ^{[1
,2
]}

Zeng, Shaoqun ^{[1
,2
]}

机构：

[1] Huazhong Univ Sci & Technol, Wuhan Natl Lab Optoelect, Britton Chance Ctr Biomed Photon, Wuhan 430074, Hubei, Peoples R China

[2] Huazhong Univ Sci & Technol, Sch Engn Sci, Collaborat Innovat Ctr Biomed Engn, MOE Key Lab Biomed Photon, Wuhan 430074, Hubei, Peoples R China

来源：

OPTICS LETTERS | 2018年 / 43卷 / 19期

基金：

中国国家自然科学基金;

关键词：

NEURONAL-ACTIVITY; MICROSCOPY; LENS; BRAIN;

D O I：

10.1364/OL.43.004598

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

The two-photon microscope is a powerful tool in life science. Conventional two-photon microscopy can image only a plane of a particular axial position at a time. Axial scanning can get the volumetric information, but it gets signals from different axial positions serially, which means that the exposure time at every plane is limited. Here we demonstrate a novel method, to the best of our knowledge, that can simultaneously record images from two planes at different xyz positions. The demultiplexing of the signal is realized using a confocal strategy. The experimental results show that it can be used for simultaneous two-photon imaging at two focal planes with little cross talk. (C) 2018 Optical Society of America

引用

收藏

页码：4598 / 4601

页数：4

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