Species-Specific Type II Restriction-Modification System of Xylella fastidiosa Temecula1

被引：21

作者：

Matsumoto, Ayumi ^{[1
]}

Igo, Michele M. ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Microbiol, Davis, CA 95616 USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 12期

关键词：

IN-VITRO METHYLATION; TRANSFORMATION EFFICIENCY; ESCHERICHIA-COLI; PIERCES-DISEASE; DNA METHYLASE; CLONING; GENES; EXPRESSION; STRAINS; BARRIER;

D O I：

10.1128/AEM.03034-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The transformation efficiency of Xylella fastidiosa can be increased by interfering with restriction by the strain-specific type II system encoded by the PD1607 and PD1608 genes. Here, we report results for two strategies: in vitro methylation using M. SssI and isolation of DNA from an Escherichia coli strain expressing the methylase PD1607.

引用

页码：4092 / 4095

页数：4

共 22 条

[21] pHluorin-assisted expression, purification, crystallization and X-ray diffraction data analysis of the C-terminal domain of the HsdR subunit of the Escherichia coli type I restriction-modification system EcoR124I
Grinkevich, Pavel
Iermak, Iuliia
Luedtke, Nicholas A.
Mesters, Jeroen R.
Ettrich, Rudiger
Ludwig, Jost
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2016, 72 : 672 - 676
[22] Medically Relevant Acinetobacter Species Require a Type II Secretion System and Specific Membrane-Associated Chaperones for the Export of Multiple Substrates and Full Virulence
Harding, Christian M.
Kinsella, Rachel L.
Palmer, Lauren D.
Skaar, Eric P.
Feldman, Mario F.
PLOS PATHOGENS, 2016, 12 (01)

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