Open sandwich immunoassay: A novel immunoassay approach based on the interchain interaction of an antibody variable region

被引：32

作者：

Ueda, H ^{[1
]}

机构：

[1] Univ Tokyo, Grad Sch Frontier Sci, Dept Integrated Biosci, Kashiwa, Chiba 2778562, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2002年 / 94卷 / 06期

关键词：

phage display; fusion protein; energy transfer; enzymatic complementation; growth control; aptamer;

D O I：

10.1016/S1389-1723(02)80203-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Despite their general applicability, conventional sandwich immunoassays suffer from the requirements of two antibodies recognizing different epitopes, and multiple long incubation/washing steps. In addition, the assay is not applicable to the measurement of low-molecular-weight haptens due to their size. Recently, a novel sandwich-type immunoassay that can overcome the limitations of conventional sandwich immunoassays was proposed. The assay, which utilizes antigen-dependent stabilization of an antibody variable region, requires less time and handling, and is particularly suitable for hapten measurement. The principle could be applied to several homogeneous assay formats, and the method has been shown to have superior sensitivity, as well as a lower time requirement compared to conventional assays.

引用

页码：614 / 619

页数：6

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