A DREPP protein interacted with PeaT1 from Alternaria tenuissima and is involved in elicitor-induced disease resistance in Nicotiana plants

被引：9

作者：

Meng, Fanlu ^{[1
]}

Xiao, Yao ^{[1
]}

Guo, Lihua ^{[1
]}

Zeng, Hongmei ^{[1
]}

Yang, Xiufen ^{[1
]}

Qiu, Dewen ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, 12 Zhongguancun South St, Beijing 100081, Peoples R China

来源：

JOURNAL OF PLANT RESEARCH | 2018年 / 131卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Disease resistance; DREPP family; Elicitor PeaT1; Protein-protein interaction; DESTABILIZING CORTICAL MICROTUBULES; CATION-BINDING PROTEIN; TOBACCO MOSAIC-VIRUS; PLASMA-MEMBRANE; PHOSPHATIDYLINOSITOL PHOSPHATES; MASS-SPECTROMETRY; DEFENSE RESPONSES; N-MYRISTOYLATION; ARABIDOPSIS; PATHOGEN;

D O I：

10.1007/s10265-018-1038-5

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

PeaT1 is a proteinaceous elicitor from fungal pathogen Alternaria tenuissima. Our previous research revealed that this elicitor could induce defense response and enhance disease resistance in various plants including Nicotiana plants. However, immune activation mechanisms whereby PeaT1 elicits defense response remain unclear. In this study, the association between elicitor protein PeaT1 and the plasma membrane was assessed using the FITC (Fluorescein isothiocyanate) labeling method. A PeaT1-interacting protein was isolated via I-125-PeaT1 cross-linking and Far Western blot analyses, and designated PtBP1 (PeaT1 Binding Protein 1). From the data of Mass spectrometry (MS) and bioinformatics analysis, the 22 kDa plasma membrane protein PtBP1 was inferred to be a member of DREPP (developmentally regulated plasma membrane polypeptide) family that is induced in plants under stress conditions and might get involved in downstream signaling. For further verification of this association, Far Western blot, co-immunoprecipitation and bimolecular fluorescence complementation (BiFC) analyses were performed, showing PtBP1 could bind with PeaT1 in vitro and in vivo. Virus-induced gene silencing (VIGS) analysis exhibited that PtBP1 silencing in Nicotiana benthamiana attenuated tobacco mosaic virus (TMV) resistance compared to the tobacco rattle virus (TRV) control after PeaT1 treatment.

引用

页码：827 / 837

页数：11