Biochar affects soil organic matter cycling and microbial functions but does not alter microbial community structure in a paddy soil

被引:223
作者
Tian, Jing [1 ]
Wang, Jingyuan [1 ]
Dippold, Michaela [2 ]
Gao, Yang [1 ]
Blagodatskaya, Evgenia [2 ,3 ]
Kuzyakov, Yakov [2 ,4 ]
机构
[1] Chinese Acad Sci, Inst Geog Sci & Nat Resources Res, Key Lab Ecosyst Network Observat & Modeling, Beijing 100101, Peoples R China
[2] Univ Gottingen, Dept Soil Sci Temperate Ecosyst, D-37077 Gottingen, Germany
[3] Russian Acad Sci, Inst Physicochem & Biol Problems Soil Sci, Pushchino 142290, Russia
[4] Univ Gottingen, Dept Agr Soil Sci, D-37077 Gottingen, Germany
基金
中国国家自然科学基金; 俄罗斯科学基金会; 国家自然科学基金重大项目;
关键词
Biochar; Soil organic matter fraction; Microbial community; Enzymes; Microbial function; FATTY-ACID PROFILES; CARBON SEQUESTRATION; ENZYME-ACTIVITY; BLACK CARBON; SANDY LOAM; NITROGEN; CHARCOAL; MANURE; FRACTIONS; DIVERSITY;
D O I
10.1016/j.scitotenv.2016.03.010
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The application of biochar (BC) in conjunction with mineral fertilizers is one of the most promising management practices recommended to improve soil quality. However, the interactive mechanisms of BC and mineral fertilizer addition affecting microbial communities and functions associated with soil organic matter (SOM) cycling are poorly understood. We investigated the SOM in physical and chemical fractions, microbial community structure (using phospholipid fatty acid analysis, PLFA) and functions (by analyzing enzymes involved in C and N cycling and Biolog) in a 6-year field experiment with BC and NPK amendment. BC application increased total soil C and particulate organic C for 47.4-50.4% and 63.7-74.6%, respectively. The effects of BC on the microbial community and C-cycling enzymes were dependent on fertilization. Addition of BC alone did not change the microbial community compared with the control, but altered the microbial community structure in conjunction with NPK fertilization. SOM fractions accounted for 55% of the variance in the PLFA-related microbial community structure. The particulate organic N explained the largest variation in themicrobial community structure. Microbial metabolic activity strongly increased after BC addition, particularly the utilization of amino acids and amines due to an increase in the activity of proteolytic (L-leucine aminopeptidase) enzymes. These results indicate that microorganisms start to mine N from the SOM to compensate for high C:N ratios after BC application, which consequently accelerate cycling of stable N. Concluding, BC in combination with NPK fertilizer application strongly affected microbial community composition and functions, which consequently influenced SOM cycling. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码:89 / 97
页数:9
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