Building the Connectivity Map of epigenetics: Chromatin profiling by quantitative targeted mass spectrometry

被引：41

作者：

Creech, Amanda L. ^{[1
]}

Taylor, Jordan E. ^{[1
]}

Maier, Verena K. ^{[2
]}

Wu, Xiaoyun ^{[1
]}

Feeney, Caitlin M. ^{[1
]}

Udeshi, Namrata D. ^{[1
]}

Peach, Sally E. ^{[1
]}

Boehm, Jesse S. ^{[1
]}

Lee, Jeannie T. ^{[2
,3
]}

Carr, Steven A. ^{[1
]}

Jaffe, Jacob D. ^{[1
]}

机构：

[1] Broad Inst MIT & Harvard Univ, Cambridge, MA 02142 USA

[2] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Boston, MA 02114 USA

[3] Harvard Univ, Sch Med, Howard Hughes Med Inst, Boston, MA 02114 USA

来源：

METHODS | 2015年 / 72卷

关键词：

Histones; Chromatin; Epigenetics; Proteomics; Mass spectrometry; SILAC; H3; LYSINE; 27; HISTONE H3; METHYLATION; EZH2; MUTATIONS;

D O I：

10.1016/j.ymeth.2014.10.033

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Epigenetic control of genome function is an important regulatory mechanism in diverse processes such as lineage commitment and environmental sensing, and in disease etiologies ranging from neuropsychiatric disorders to cancer. Here we report a robust, high-throughput targeted, quantitative mass spectrometry (MS) method to rapidly profile modifications of the core histones of chromatin that compose the epigenetic landscape, enabling comparisons among cells with differing genetic backgrounds, genomic perturbations, and drug treatments. (C) 2014 ElsevierInc. All rights reserved.

引用

页码：57 / 64

页数：8

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