MICROVASCULAR AND METABOLIC EFFECTS OF A BALANCED ELECTROLYTE SOLUTION AND HEPARINIZED AUTOLOGOUS BLOOD IN HEMORRHAGIC SHOCK

Aim: To compare the microvascular and metabolic effects of autologous blood and a balanced electrolyte solution (BES) containing acetate and malate in hemorrhagic shock (HS) in rats. Methods. The experiments were carried out on male Wistar rats (n=21) under general anesthesia. Blood (35% of estimated blood volume) was withdrawn for 5 minutes. After 60 minutes of HS the collected blood was retransfused, followed by the infusion of BES in the volume of 50% of the blood loss (HS+AB group); HS+BES group received BES in a triple volume of blood loss; a sham-hemorrhage control. Heart rate (HR), mean arterial pressure (MAP), skin blood flow (M), gases and acid-base state of arterial blood, post-occlusive reactive hyperemia (PORH) in the rat tail, serum levels of biomarkers S100b and NSE were assessed after 24 hours. After euthanasia, a histological examination of the brain, lungs, heart and kidneys was performed. Results. In HS+AB and HS+BES groups, 24-hour mortality did not differ (one animal in each group). MAP in these groups was moderately reduced compared to sham group. There were no significant differences in HR, M, acid-base state, PORH and levels of S100b and NSE between the groups. Histological examination revealed circulatory disorders of varying severity in groups with hemorrhage, with more pronounced damage to the brain and kidneys in HS+BES group. Conclusion. Both autologous blood and BES containing acetate and malate equally restore MAP, skin microcirculation, and acid-base state 24 hours after HS. However, fluid resuscitation with BES alone is associated with brain and kidney damage.