Fluorescent fullerene nanoparticle-based lateral flow immunochromatographic assay for rapid quantitative detection of C-reactive protein

被引：19

作者：

Park, Kyung Mi ^{[1
]}

Chung, Da Jung ^{[1
]}

Choi, Mijin ^{[1
]}

Kang, Taejoon ^{[2
,3
]}

Jeong, Jinyoung ^{[3
,4
]}

机构：

[1] Korea Res Inst Biosci & Biotechnol, BioNano Hlth Guard Res Ctr, Daejeon 34141, South Korea

[2] Korea Res Inst Biosci & Biotechnol, Bionanotechnol Res Ctr, Daejeon 34141, South Korea

[3] Univ Sci & Technol, KRIBB Sch Biotechnol, Dept Nanobiotechnol, Daejeon 34113, South Korea

[4] Korea Res Inst Biosci & Biotechnol, Environm Dis Res Ctr, Daejeon 34141, South Korea

来源：

NANO CONVERGENCE | 2019年 / 6卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

Fullerene; Nanoparticle; C-reactive protein; Lateral flow immunochromatographic assay; Fluorescence; SENSITIVE DETECTION; IMMUNOASSAY; POINT; GOLD; STRIPS;

D O I：

10.1186/s40580-019-0207-0

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

A fluorescent fullerene nanoparticle (NP)-based lateral flow immunochromatographic assay (LFIA) was developed for the rapid and quantitative detection of C-reactive protein (CRP) in serum. The polyclonal CRP-antibody-conjugated fullerene NPs were simply prepared by 1-ethyl-3-(3-dimethyllaminopropyl)-carbodiimide hydrochloride coupling after carboxylation of fluorescent fullerene NPs. By applying the CRP-antibody-conjugated fullerene NPs to a lateral flow test strip, quantitative analysis of CRP in serum was possible at a concentration range of 0.1-10 ng/ml within 15 min. We anticipate that this novel fluorescent fullerene NP-based LFIA can be useful for the rapid and accurate sensing of biological and chemical species, contributing to the disease diagnosis and prognosis, environmental monitoring, and food safety.

引用

页数：6

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