Thermo-responsive properties of methylcellulose hydrogels for cell sheet engineering

被引：59

作者：

Contessi, Nicola ^{[1
,2
]}

Altomare, Lina ^{[1
,2
]}

Filipponi, Andrea ^{[1
]}

Fare, Silvia ^{[1
,2
]}

机构：

[1] Politecn Milan, Dept Chem Mat & Chem Engn G Natta, Piazza Leonardo da Vinci 32, I-20133 Milan, IT, Italy

[2] Natl Interuniv Consortium Mat Sci & Technol, INSTM, Florence, Italy

来源：

MATERIALS LETTERS | 2017年 / 207卷

关键词：

Smart materials; Methylcellulose; Rheology; UV spectroscopy; Cell sheet engineering; GELATION;

D O I：

10.1016/j.matlet.2017.07.023

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Methylcellulose (MC) hydrogels change their affinity to water depending on their temperature and can thus be used as substrates for cell sheet engineering. In this work, we characterize the thermo-responsive properties of 8% w/v MC hydrogels, produced in two saline solutions (i.e., Na2SO4 and phosphate buffered saline) at different concentrations, by investigating the rheological properties and the UV-absorbance in function of temperature. Both rheological and UV-spectroscopy tests showed that the addition of salts to MC hydrogels allowed lowering the LCST of the MC hydrogel; moreover, hydrogels produced in 0.1 M Na2SO4 or PBS 20 g/L were proved to be particularly promising for cell sheet engineering application, showing a LCST below 37 degrees C. (C) 2017 Elsevier B.V. All rights reserved.

引用

页码：157 / 160

页数：4

共 11 条

[1] Thermo-responsive methylcellulose hydrogels as temporary substrate for cell sheet biofabrication [J].

Altomare, Lina ;

Cochis, Andrea ;

Carletta, Andrea ;

Rimondini, Lia ;

Fare, Silvia .

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE, 2016, 27 (05)

[2] Synergistic effect of salt mixture on the gelation temperature and morphology of methylcellulose hydrogel [J].

Bain, Mrinal Kanti ;

Bhowmick, Biplab ;

Maity, Dipanwita ;

Mondal, Dibyendu ;

Mollick, Md Masud Rahaman ;

Rana, Dipak ;

Chattopadhyay, Dipankar .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2012, 51 (05) :831-836

[3]

Forghani A., 2017, J BIOMED MATER RES A, P1

[4] A thermorheological investigation into the gelation and phase separation of hydroxypropyl methylcellulose aqueous systems [J].

Hussain, S ;

Keary, C ;

Craig, DQM .

POLYMER, 2002, 43 (21) :5623-5628

[5]

Jain S., 2013, Journal of Applied Pharmaceutical Science, V3, P139, DOI DOI 10.7324/JAPS.2013.31225

[6] Human Adipose Tissue Derived Extracellular Matrix and Methylcellulose Hydrogels Augments and Regenerates the Paralyzed Vocal Fold [J].

Kim, Dong Wook ;

Kim, Eun Ji ;

Kim, Eun Na ;

Sung, Myung Whun ;

Kwon, Tack-Kyun ;

Cho, Yong Woo ;

Kwon, Seong Keun .

PLOS ONE, 2016, 11 (10)

[7] Thermoresponsive hydrogels in biomedical applications A seven-year update [J].

Klouda, Leda .

EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS, 2015, 97 :338-349

[8] KINETICS OF THERMAL GELATION OF METHYLCELLULOSE AND HYDROXYPROPYLMETHYLCELLULOSE IN AQUEOUS-SOLUTIONS [J].

SARKAR, N .

CARBOHYDRATE POLYMERS, 1995, 26 (03) :195-203

[9] Thermoreversible and salt-sensitive turbidity of methylcellulose in aqueous solution [J].

Xu, Y ;

Li, L .

POLYMER, 2005, 46 (18) :7410-7417

[10] Controllable gelation of methylcellulose by a salt mixture [J].

Xu, YR ;

Li, L ;

Zheng, PJ ;

Lam, YC ;

Hu, X .

LANGMUIR, 2004, 20 (15) :6134-6138

← 1 2 →