Kinetics of unfolding the human telomeric DNA quadruplex using a PNA trap

被引：94
|
作者：
Green, JJ [1 ]
Ying, LM [1 ]
Klenerman, D [1 ]
Balasubramanian, S [1 ]
机构：
[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England
来源：
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2003年 / 125卷 / 13期
关键词：
D O I：
10.1021/ja029149w
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
The kinetics of opening of the DNA quadruplex formed by the human telomeric repeat have been investigated using real-time fluorescence resonance energy transfer (FRET) measurements with a peptide nucleic acid (PNA) trap. It has been found that this opening is zero-order with respect to PNA, indicating that the initial step is a rate-limiting internal rearrangement of the quadruplex. A study of the temperature dependence of the rate of quadruplex opening was performed and the activation energy of the process estimated to be 98 +/- 8 kJ mol(-1).
引用
收藏
页码：3763 / 3767
页数：5
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