Drosophila IRBP/Ku p70 corresponds to the mutagen-sensitive mus309 gene and is involved in P-element excision in vivo

被引:92
作者
Beall, EL [1 ]
Rio, DC [1 ]
机构
[1] UNIV CALIF BERKELEY,DEPT MOLEC & CELL BIOL,BERKELEY,CA 94720
关键词
DNA repair; P elements; transposition; Drosophila; mutagen sensitivity;
D O I
10.1101/gad.10.8.921
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The P family of transposable elements in Drosophila transpose by a cut-and-paste mechanism involving double-strand gap repair. We report here that a Drosophila mutagen-sensitive mutant, mus309, contains a mutation in IRBP (inverted repeat binding protein), the Drosophila homolog of the mammalian Ku p70 gene. We show that the repair of double-strand DNA breaks after P-element excision is severely reduced in mus309 mutants using an in vivo assay for P-element transposase activity. In addition, excision products recovered from mus309 mutant embryos by use of a plasmid-based P-element mobility assay contain large deletions, suggesting that IRBP is involved in the repair of double-strand DNA breaks. Our findings provide the first demonstration that a mutation in the IRBP gene affects double-strand DNA break repair and suggest that DNA repair functions are conserved between Drosophila and mammals.
引用
收藏
页码:921 / 933
页数:13
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