Toward an Ensemble View of Chromatosome Structure: A Paradigm Shift from One to Many

被引：26

作者：

Ozturk, Mehmet Ali ^{[1
,2
]}

Cojocaru, Vlad ^{[3
,4
]}

Wade, Rebecca C. ^{[1
,5
,6
]}

机构：

[1] HITS, Mol & Cellular Modeling Grp, D-69118 Heidelberg, Germany

[2] Heidelberg Univ, Hartmut Hoffmann Berling Int Grad Sch Mol & Cellu, D-69120 Heidelberg, Germany

[3] Max Planck Inst Mol Biomed, Dept Cellular & Dev Biol, Computat Struct Biol Lab, D-48149 Munster, Germany

[4] Westfalische Wilhelms Univ, Ctr Multiscale Theory & Computat, D-48149 Munster, Germany

[5] Heidelberg Univ, DKFZ ZMBH Alliance, Ctr Mol Biol ZMBH, D-69120 Heidelberg, Germany

[6] Interdisciplinary Ctr Sci Comp IWR, D-69120 Heidelberg, Germany

来源：

STRUCTURE | 2018年 / 26卷 / 08期

关键词：

LINKER HISTONE H1; IN-VIVO; MOUSE DEVELOPMENT; CORE PARTICLE; NUCLEOSOME; DNA; BINDING; SUBTYPES; COMPLEX; FIBER;

D O I：

10.1016/j.str.2018.05.009

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

There is renewed interest in linker histone (LH)-nucleosome binding and how LHs influence eukaryotic DNA compaction. For a long time, the goal was to uncover "the structure of the chromatosome,'' but recent studies of LH-nucleosome complexes have revealed an ensemble of structures. Notably, the reconstituted LH-nucleosome complexes used in experiments rarely correspond to the sequence combinations present in organisms. For a full understanding of the determinants of the distribution of the chromatosome structural ensemble, studies must include a complete description of the sequences and experimental conditions used, and be designed to enable systematic evaluation of sequence and environmental effects.

引用

页码：1050 / 1057

页数：8