Comparative assessment of chemical and γ-irradiation procedures for implantable glucose enzyme electrodes

被引:6
作者
Ahmed, S [1 ]
Rigby, GP [1 ]
Crump, P [1 ]
Vadgama, PM [1 ]
机构
[1] Hope Hosp, Clin Biochem Sect, Salford M6 8HD, Lancs, England
关键词
needle glucose sensors; sterilisation; irradiation;
D O I
10.1016/S0956-5663(00)00057-9
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Chemical and gamma-irradiation sterilisation were examined in this study for implantable needle electrodes. Exposure to isopropyl alcohol (IPA) led to response elevation, but time-dependent exposures up to 30 min variously to chlorhexidine, H2O2, HCl, HCl/IPA, and alcoholic iodine/potassium iodide, all caused substantial time-dependent response degradation. Sterility was not assessed for such electrodes. High dose (30 KGy) gamma-irradiation also compromised sensor response, with exposed electrodes exhibiting similar to 30% reduction in response, comparable in magnitude to 10 min exposure to chemical sterilisation. However, a lower dose (25 KGy) gamma-irradiation was well tolerated and subsequent microbiological testing (following contamination with Streptococcus epidermidis and Staphylococus aureus) proved device sterility with no culture growth following 7 days incubation at 37 degrees C in brain heart infusion medium. (C) 2000 Elsevier Science S.A. All rights reserved.
引用
收藏
页码:159 / 165
页数:7
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